Hepatitis C Qualitative PCR, Tissue

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General Information

Lab Name
Hepatitis C Qualitative PCR, Tissue
Lab Code
HCVQLT
Epic Ordering
Hepatitis C Qualitative PCR, Tissue
Description

The Abbott real-time FDA approved HCV assay detects and quantifies Hepatitis C virus by real-time PCR amplification with primers specific for the 5’UTR region . The RNA concentration detected with a probability of 95% or greater is 12 IU/mL (1.08 log IU/mL).

For Blood samples, see Hepatitis C RNA, Quantitative [HCVQNT].

For HCV genotyping, see Hepatitis C RNA Genotyping by PCR [HCPCGT].

Synonyms
HCV RNA Qualitative, Hepatitis C by PCR, PCR-Hepatitis C
Components

Interpretation

Method

Real-time Polymerase Chain Reaction.

Reference Range
See individual components

Ordering & Collection

Specimen Type
Tissue Biopsy, Bone Marrow, Swab or Cellular fluid (ex: BAL or Washing)
Collection

Tissue Biopsy: Pea Size in sterile tube/universal transport media.

Bone Marrow: 5 mL blood in EDTA (LAVENDER TOP) tube, No Heparin aspirate in syringe.

Swab: In sterile tube/universal transport media (UTM).

Cellular fluid: 1 mL in sterile tube

Unacceptable samples: Buffy coat collected with heparin syringe. Paraffin samples.

Handling Instructions

Bone Marrow: Send LAVENDER TOP to Laboratory at room temperature within 24 hour of collection.


Note: Separate specimens MUST be submitted when both Viral Culture and PCR testing are ordered.

Quantity
requested: See Collection
minimum: 0.5 mL

Processing

Processing

Tissue Biopsy: Freeze at -10°C or colder. Ship on dry ice. Sample may be stored at 2-8°C (ice pack) within 48 hours of collection. Do not freeze/thaw.

Bone Marrow: Ship at room temperature within 24 hours of collection.

Swabs: Freeze at -10°C or colder. Ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Cellular fluid: Freeze at -10°C or colder. Ship frozen samples on dry ice. Sample may be stored at 2-8°C (ice pack) for up to 48 hours from collection. Do not freeze/thaw.

Outside Laboratories:

Buffy coat: Harvest buffy coat from Bone Marrow within 24 hours of collection, Freeze at -10°C or colder.

Tissue/Cellular fluid/Swab: Ship samples on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Shipment time must not exceed 48 hours from collection. Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Performance

LIS Dept Code
Virology, Eastlake (EVIR)
Performing Location(s)
Other Virology, Molecular
206-685-6656

Virology Laboratory, Ste 320
1616 Eastlake Ave E.
Seattle, WA 98102
Frequency
Wednesday. Results following Thursday.
Available STAT?
No

Billing & Coding

CPT codes
87521
LOINC
5011-2
Interfaced Order Code
UOW3763