Hepatitis C Antibody without PCR reflex on reactive samples
General Information
- Lab Name
- Hepatitis C Ab w/o PCR
- Lab Code
- HCABX
- Epic Ordering
- Hepatitis C Antibody w/o PCR
- Description
The Qualitative detection of Hepatitis C virus IgG and IgM antibodies (anti-HCV) in human sera by the FDA approved Abbott ARCHITECT Anti-HCV test two-step chemiluminescent immunoassay.
In the first step, sample, assay diluent, and recombinant HCV antigen (rHCV) coated paramagnetic microparticles are combined. Anti-HCV present in the sample binds to the rHCV coated microparticles. In the second step, anti-human IgG/IgM acridinium-labeled conjugate is added, which binds to IgG and IgM anti-HCV. Then pre-trigger and trigger solutions are added to the reaction mixture. The resulting chemiluminescent reaction is measured as relative light units (RLUs).
The presence or absence of IgG/IgM anti-HCV in the sample is determined by comparing the chemiluminescent signal in the reaction to the cutoff signal determined from an ARCHITECT Anti-HCV calibration. Specimens with signal to cutoff (S/CO) values ≥ 1.00 are considered reactive for IgG/IgM anti-HCV. Specimens with S/CO values < 0.79 are considered nonreactive and specimens with S/CO values between 0.80 and 0.99 are Indeterminate.
For anti-HCV testing with PCR reflex for REACTIVE results, see Hepatitis C Antibody with Reflex to PCR [HCAB]. Reactive anti-HCV will reflex to Hepatitis C RNA, Quantitative [HCVQNT] for confirmation with an additional charge.
- Synonyms
- Components
-
Code Name HCABRX Hepatitis C Ab Result w/o PCR IN46 Hepatitis C Ab Interp
Interpretation
- Method
Chemiluminescence Immunoassay
- Reference Range
- See individual components
- Guidelines
Ordering & Collection
- Specimen Type
- Blood
- Collection
-
Blood: 5 mL blood in GOLD TOP serum separator tube (SST).
Also acceptable: 5 mL blood in ORANGE TOP rapid serum tube (RST) or RED TOP tube
- Handling Instructions
- Quantity
-
requested: 1.0 mL serum
minimum: 0.5 mL serum
Processing
- Processing
Blood: Specimens may be stored on or off the clot, red blood cells, or separator gel for up to 3 days at room temperature (15-30°C) or up to 7 days at 2-8°C. If testing will be delayed more than 3 days for specimens stored at room temperature or more than 7 days for specimens stored at 2-8°C, remove serum from the clot, red blood cells, or separator gel and store at -20°C or colder. Avoid more than three freeze/thaw cycles.
Outside Laboratories: Specimens may be stored on or off the clot, red blood cells, or separator gel for up to 3 days at room temperature (15-30°C) or up to 7 days at 2-8°C. If testing will be delayed more than 3 days for specimens stored at room temperature or more than 7 days for specimens stored at 2-8°C, remove serum from the clot, red blood cells, or separator gel and store at -20°C or colder. Avoid more than three freeze/thaw cycles.
Unacceptable samples: Cord blood, neonatal specimens, cadaver specimens, heat-inactivated specimens, or body fluids such as saliva, urine, amniotic fluid, or pleural fluid. Specimens with obvious microbial contamination or are grossly hemolyzed.
Performance
- LIS Dept Code
- Clinical Virology (CVIR)
- Performing Location(s)
-
Other Virology
206-685-8037Virology Laboratory, Ste 320
1616 Eastlake Ave E.
Seattle, WA 98102 - Frequency
- Monday-Friday
- Available STAT?
- No
Billing & Coding
- CPT codes
- 86803
- LOINC
- 40726-2
- Interfaced Order Code
- UOW1908