Hepatitis B Qualitative PCR, Tissue
General Information
- Lab Name
- Hepatitis B Qualitative PCR, Tissue
- Lab Code
- HBVQLT
- Epic Ordering
- Hepatitis B Qualitative PCR, Tissue
- Description
The detection HBV by real-time PCR amplification. The primers used in this PCR reaction amplifies the Polymerase region gene and the probe hybridizes the target sequences between the primers that specifically identifies the PCR amplicon. The Quantification of HBV DNA (IU/mL) is achieved by amplifying in each run standard curves consisting of serial dilutions of a known number of DNA containing the HBV amplicons. Results are reported in International Units per milliliter (IU/mL). The minimum virus level that gives a positive result in 95% of replicates is 25 IU/mL (1.4 log IU/mL).
This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration.
For Blood, see Hepatitis B Quantitative [HBVQNT].
- Synonyms
- HBV DNA Qualitative
- Components
-
Code Name HBVQLS Hepatitis B Qualitative PCR Specimen Type HBVQLR Hepatitis B Qualitative PCR Result HBVQLI Hepatitis B Qualitative PCR Interpretation
Interpretation
- Method
Real-time Polymerase Chain Reaction
- Reference Range
- See individual components
Ordering & Collection
- Specimen Type
- Tissue Biopsy, Paraffin Tissue, Bone Marrow, Swab or Cellular fluid (ex: BAL or Washing)
- Collection
-
Tissue Biopsy: Pea size amount in sterile tube or universal transport media (UTM).
Paraffin Tissue: DO NOT SEND ENTIRE BLOCK and DO NOT SEND SLIDES. Slice 3-4 slices that are 5-50 micron thick in sterile tube.
Bone Marrow: 5 mL blood in EDTA (LAVENDER TOP) tube, No Heparin aspirate in syringe.
Swab: In sterile tube/universal transport media (UTM).
Cellular fluid: 1 mL in sterile tube.
Unacceptable samples: Buffy coat collected with heparin syringe. Paraffin slides.
- Handling Instructions
Bone Marrow: 5 mL bone marrow in EDTA (LAVENDER TOP) tube to Laboratory at room temperature within 24 hour of collection.
Note: Separate specimens MUST be submitted when both Viral Culture and PCR testing are ordered.
- Quantity
-
requested: See Collection
minimum: 0.5 mL
Processing
- Processing
Tissue Biopsy: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.
Paraffin Tissue: Ship at room temperature.
Bone Marrow: Ship at room temperature within 24 hours of collection.
Swabs: Freeze at -10°C or colder. Ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.
Cellular fluid: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.
Outside Laboratory:
Buffy coat: Separate buffy coat from bone marrow within 24 hours of collection, Freeze at -10°C or colder.
Tissue/Cellular fluid/Swab: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Shipment time must not exceed 48 hours from collection if stored at 2-8°C. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.
Performance
- LIS Dept Code
- Virology, Eastlake (EVIR)
- Performing Location(s)
-
Other Virology, Molecular
206-685-6656Virology Laboratory, Ste 320
1616 Eastlake Ave E.
Seattle, WA 98102 - Frequency
- Wednesday. Results following Friday.
- Available STAT?
- No
Billing & Coding
- CPT codes
- 87516
- LOINC
- 5008-8
- Interfaced Order Code
- UOW3759