Hemoglobin Electrophoresis (Isoelectric Focusing)

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General Information

Lab Name
Hb Elect._Isoelectric Focusing
Lab Code
HBELEC
Epic Name
Hemoglobin Electrophoresis (Isoelectric Focusing)
Description

Hemoglobin electrophoresis alone is not adequate for diagnosing thalassemia. Electrophoresis is limited to identification without fractionation, quantification or interpretation. This test is useful for screening for sickle cell trait for athletes other than those on UW teams. For most red cell disorders, we suggest instead ordering "Hb Disorder Thalassemia Reflex Pnl w/CBC" [HBTHLI]. If a CBC was performed within 30 days, order "Hb Disorder Thalassemia Reflexive Panel" [HBTHAL]. Both panels include hb electrophoresis, reflexive testing for thalassemia and an interpretation.

Synonyms
HB ELP, Hemoglobinopathy, hemoglobinopathy, red cell, Sickle Cell Trait, Sickle Cell-Routine, Sickle Dex-Routine
Components
Code Name
HER Hb Electrophoresis Result
HBDET Hemoglobins Detected
HBCOM Hb Elect. Comment

Interpretation

Guidelines
Method

Isoelectric Focusing Electrophoresis (IEF)

Reference Range
See individual components
Ref. Range Notes

Within Normal Limits

Ordering & Collection

Specimen Type
Blood
Collection

3 mL LAVENDER HEMOGARD tube

Pediatric draw: 3 LAVENDER TOP MICROTAINER

Handling Instructions

For STAT requests from ED location, see [SICPRE] Sickle Screen, Stat, ED only.

Quantity
Requested: 3 mL whole blood
Minimum: 1.5 mL whole blood

Processing

Receiving Instructions

Refrigerate entire sample. If sickle screen is ordered, but not from ED location, login HBELEC.

If request is for Quantitative HbS or Hb Variant Quant., for pre- or post-transfusion, login HBQVAR.

If request is for Quantitative HBF for hydroxyurea therapy, login HBF.

Stability: EDTA: Refrigerated, 7 days. Ambient, 2 days

Unacceptable: Other anticoagulants, Frozen specimen

Misc Sendout

Performance

Lab Department
Red Cell Disorders(RCD)
Frequency
Monday - Friday
Available STAT?
No
Performing Location(s)
HMC Red Cell Disorders
206-520-4600

325 9th Ave, Rm # GWH-47, Seattle, WA 98104-2420

Billing & Coding

CPT Codes
83020
LOINC
43113-0
Interfaced Order Code
UOW414