Hepatitis B Envelope Antigen and Antibody
General Information
- Lab Name
- Hepatitis B e Ag and Ab by EIA
- Lab Code
- HBE
- Epic Name
- Hepatitis B Envelope Antigen and Antibody
- Description
The Qualitative detection of Hepatitis B virus “e” antigen (HBeAg) and Hepatitis B virus “e” antibody (HBeAb) in human sera by the FDA approved LIASON® IgG Assay.
The presence of HBeAg in serum is associated with presence of Dane particles and Hepatitis B virus (HBV) DNA polymerase enzyme. These markers suggest active HBV replication. Hepatitis B Surface antigen (HBsAg) positive and HBeAg positive persons are much more likely to transmit infections to others. The development of anti-HBe during acute Hepatitis B infection is a marker of resolution of illness.
- Components
-
Code Name HEAG Hepatitis B e Ag HEAB Hepatitis B e Ab IN3 Hepatitis B e Ag and Ab Interp
Interpretation
- Guidelines
- Method
The qualitative determination of HBeAg is a direct, sandwich chemiluminescence immunoassay (CLIA). The qualitative determination of anti-HBe is a competitive sandwich chemiluminescence immunoassay (CLIA) based on neutralization.
- Reference Range
- See individual components
Ordering & Collection
- Specimen Type
- Blood
- Collection
-
Blood: 5 mL blood in GOLD TOP serum separator tube (SST).
Also acceptable: 5 mL blood in ORANGE TOP rapid serum tube (RST), RED TOP tube or the following anticoagulants EDTA, heparin, or citrate.
- Quantity
-
Requested: 2 mL serum or plasma
Minimum: 0.6 mL serum or plasma
Processing
- Receiving Instructions
Blood: Harvest serum/plasma from whole blood within 24 hour or collection. Serum/plasma may be stored at 2-8°C (ice pack) for up to 3 days at room temperature (15-30°C) or up to 7 days. Freeze at -20°C or colder for long term storage. Do not freeze/thaw.
Outside Laboratories: Ship samples on Dry Ice, do not freeze/thaw. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack), shipment time must not to exceed 7 days from collection. Samples stored at 2-8°C greater than 7 days old will be rejected.
Unacceptable samples: Cord blood, neonatal specimens, cadaver specimens, heat-inactivated specimens, or body fluids such as saliva, urine, amniotic fluid, or pleural fluid. Specimens with obvious microbial contamination, lipemic or are grossly hemolyzed.
Performance
- Lab Department
- Clinical Virology(CVIR)
- Frequency
- Once per week.
- Available STAT?
- No
- Performing Location(s)
-
Other Virology
206-685-8037Virology Laboratory, Ste 320
1616 Eastlake Ave E.
Seattle, WA 98102
Billing & Coding
- CPT Codes
- 86707, 87350
- LOINC
- 18719-5
- Interfaced Order Code
- UOW1183