Hemoglobin A2 Quantification by HPLC

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General Information

Lab Name
Hemoglobin A2 Quant
Lab Code
HBA2
Epic Ordering
Hemoglobin A2 Quant
Description

Hb A2 is eluted from the column resin by a programmed buffer gradient of increasing ionic strength. Hb A2 measurement is best interpreted in conjunction with other testing in the Thalassemia Reflexive Panel and red cell indices and iron supply measurements.

Synonyms
HbA2, Hb A2

Interpretation

Method

High Performance Liquid Chromatography (HPLC)

Reference Range
Units: %
Female Male
AgeRangeAgeRange
0-6m<3.5 0-6m<3.5
7m-2.3-3.5 7m-2.3-3.5

Effective date: 11/02/2012

Interferences and Limitations

Variant hemoglobins such as Hb S, Hb C, Hb D interfere with measurement of Hb A2, so Hb A2 is not reported in the presence of these variants. Hb E co-elutes with Hb A2 and thus the level reported is the sum of percentages of Hb E and Hb A2.

Guidelines

Ordering & Collection

Specimen Type
Blood
Collection

3 mL LAVENDER HEMOGARD tube
Pediatric draw: 3 LAVENDER TOP MICROTAINER

Handling Instructions
Quantity
requested: 3 mL whole blood
minimum: 1.5 mL whole blood

Processing

Processing

Refrigerate whole blood Stability: EDTA: Refrigerated, 7 days. Ambient, 2 days.

Unacceptable: Other anticoagulants, Frozen samples.

Performance

LIS Dept Code
Red Cell Disorders (RCD)
Performing Location(s)
HMC Red Cell Disorders
206-520-4600

325 9th Ave, Rm # GWH-47, Seattle, WA 98104-2420

Frequency
Monday - Friday
Available STAT?
No

Billing & Coding

CPT codes
83021
LOINC
42245-1
Interfaced Order Code
UOW737
Interfaced Result Code
UOW737