Hepatitis A Acute Ab (IgM)

General Information

Lab Name

Lab Code

HAVIGM

Epic Name

Hepatitis A Acute Antibody (IgM)

Description

The Qualitative detection of Hepatitis A virus IgM antibody (anti-HAV IgM) in human sera using the FDA approved Abbott ARCHITECT HAVAB-M test two-step chemiluminescent immunoassay.

In the first step, sample, assay diluent, and hepatitis A virus (human) coated paramagnetic microparticles are combined. IgM anti-HAV present in the sample binds to the hepatitis A virus (human) coated microparticles. In the second step, anti-human IgM acridinium-labeled conjugate is added, which binds to IgM anti-HAV. Then pre-trigger and trigger solutions are added to the reaction mixture. The resulting chemiluminescent reaction is measured as relative light units (RLUs).

The presence or absence of IgM anti-HAV in the sample is determined by comparing the chemiluminescent signal in the reaction to the cutoff signal determined from an ARCHITECT HAVAB-M calibration. Specimens with signal to cutoff (S/CO) values ≥ 1.21 are considered reactive for IgM anti-HAV. Specimens with S/CO values of 0.80 to < 1.21 are considered indeterminate and specimens with S/CO values < 0.80 are considered nonreactive.

Synonyms

Acute Hepatitis A, AHS, HABB, HABC, HAS, Hepatitis A antibody

Components

Code	Name
HAMAR	Hepatitis A IgM antibody
HAMIN	Hepatitis A IgM Interpretation

Interpretation

Guidelines

Washington DOH Notifiable Conditions

Method

Chemiluminescence Immunoassay

Reference Range

See individual components

Ordering & Collection

Specimen Type: Blood
Collection: Blood: 5 mL blood in GOLD TOP serum separator tube (SST).

Also acceptable: 5 mL blood in ORANGE TOP rapid serum tube (RST) or RED TOP tube
Quantity: Requested: 1.0 mL serum
Minimum: 0.5 mL serum

Processing

Receiving Instructions: Blood: Specimens may be stored on or off the clot, red blood cells, or separator gel for up to 3 days at room temperature (15-30°C) or up to 7 days at 2-8°C. If testing will be delayed more than 3 days for specimens stored at room temperature or more than 7 days for specimens stored at 2-8°C, remove serum from the clot, red blood cells, or separator gel and store at -20°C or colder. Avoid more than three freeze/thaw cycles.

Outside Laboratories: Specimens may be stored on or off the clot, red blood cells, or separator gel for up to 3 days at room temperature (15-30°C) or up to 7 days at 2-8°C. If testing will be delayed more than 3 days for specimens stored at room temperature or more than 7 days for specimens stored at 2-8°C, remove serum from the clot, red blood cells, or separator gel and store at -20°C or colder. Avoid more than three freeze/thaw cycles.

Unacceptable samples: Cord blood, neonatal specimens, cadaver specimens, heat-inactivated specimens, or body fluids such as saliva, urine, amniotic fluid, or pleural fluid. Specimens with obvious microbial contamination or are grossly hemolyzed.

Misc Sendout

Performance

Lab Department

Central Lab Virology(RVIR)

Frequency

Monday-Friday

Available STAT?

Performing Location(s)

CL	Virology, Renton 206-685-6656 1602 Raymond Ave SW Renton, WA 98057-3356	206-685-6656 opt 4

Billing & Coding

CPT Codes: 86709
LOINC: 5181-3
Interfaced Order Code: UOW3285