Testosterone, Total and Free (Patients => 18 yrs)

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General Information

Lab Name
Testosterone, Free and Total (Adults >=18y)
Lab Code
FTTEST
Epic Name
Testosterone Free and Total
Description

Do not order for patients <18 years. Order RTESFT for this group of patients.

Synonyms
Testosterone, Testosterone Free by Calculation
Components
Code Name
SHBG Sex Hormone Binding Globulin
TESTF Free Testosterone, Calculated
TESTMS Testosterone
TESTMD Testosterone by LCMS Method

Interpretation

Method

Chemiluminescence, Beckman DxI instrument (SHBG), dye-binding and spectrophotometry (albumin), and LC-MS/MS (testosterone)

Reference Range
See individual components
Ref. Range Notes

Male Reference Ranges:

  • 18y: 54.0-218.0 pg/mL
  • 19y: 53.6-212.0 pg/mL
  • 20y-24y: 52.5-207.0 pg/mL
  • 25y-29y: 50.5-198.0 pg/mL
  • 30y-34y: 48.5-190.0 pg/mL
  • 35y-39y: 46.5-181.0 pg/mL
  • 40y-44y: 44.6-171.0 pg/mL
  • 45y-49y: 42.6-164.0 pg/mL
  • 50y-54y: 40.6-156.0 pg/mL
  • 55y-59y: 38.7-147.0 pg/mL
  • 60y-64y: 36.7-139.0 pg/mL
  • 65y-69y: 34.7-130.0 pg/mL
  • 70y-74y: 32.8-122.0 pg/mL
  • 75y-79y: 30.8-113.0 pg/mL
  • 80y-84y: 28.8-105.0 pg/mL
  • 85y-89y: 26.9-96.1 pg/mL
  • 90y-94y: 24.9-87.6 pg/mL
  • 95y-: 22.9-79.1 pg/mL

Female Reference Ranges:

  • 18y: 0.0-10.9 pg/mL
  • 19y-24y: 0.6-10.8 pg/mL
  • 25y-29y: 0.6-10.6 pg/mL
  • 30y-34y: 0.6-10.3 pg/mL
  • 35y-39y: 0.6-10.0 pg/mL
  • 40y-44y: 0.6-9.8 pg/mL
  • 45y-49y: 0.6-9.5 pg/mL
  • 50y-54y: 0.6-9.2 pg/mL
  • 55y-59y: 0.6-9.0 pg/mL
  • 60y-64y: 0.6-8.7 pg/mL
  • 65y-69y: 0.6-8.4 pg/mL
  • 70y-74y: 0.6-8.2 pg/mL
  • 75y-79y: 0.6-7.9 pg/mL
  • 80y-84y: 0.6-7.6 pg/mL
  • 85y-89y: 0.6-7.3 pg/mL
  • 90y-94y: 0.6-7.1 pg/mL
  • 95y-: 0.6-6.8 pg/mL

Note: Free Testosterone concentrations for patients undergoing gender-affirming treatment should be interpreted based on patient goals and physical response. The reference ranges provided may not align with the target goals for an affirmed gender.

Ordering & Collection

Specimen Type
Blood
Collection

All Locations:

PLEASE NOTE THAT TWO TUBES ARE REQUIRED FOR THIS TESTING
Also note that a gel-free tube is required for testosterone testing.

Albumin & SHBG

Preferred: 4 mL blood in LIME GREEN TOP PST tube

Also Acceptable: 4 mL blood in GOLD TOP SST, RED TOP, ORANGE TOP SST

Testosterone by LC-MS/MS

Preferred: 4 mL blood in RED TOP

Also Acceptable: 4 mL blood in LAVENDER TOP

Pediatric: RED TOP MICROTAINER

Handling Instructions

The Laboratory MUST process testosterone specimens within 24 hours of blood collection.

Quantity
Requested: 1 mL serum/plasma
Minimum: 0.5 mL serum/plasma

Processing

Receiving Instructions

Centrifuge and separate serum or plasma for testosterone testing within 24 hours.

Refrigerate serum.

[Albumin used in Calc, but not reported.]

Note:For clinical patients <18 years, see: RTESFT. Remember we do NOT send out Research samples.

Misc Sendout

Performance

Lab Department
Chemistry, University(CHU)
Frequency
Three times each week, results expected in 3-4 days.
Available STAT?
No
Performing Location(s)
UW-MT Chemistry, Automated
206-520-4600

Clinical Lab, Room NW220,
University of Washington Medical Center,
1959 NE Pacific street, Seattle, WA 98195

Billing & Coding

CPT Codes
84270, 84403
LOINC
21555-8
Interfaced Order Code
UOW1044