Direct Antiglobulin Test
General Information
- Lab Name
- Direct Antiglobulin Test
- Lab Code
- DAT
- Epic Name
- Direct Antiglobulin Test
- Description
The Direct Antiglobulin Test (DAT) is a hemagglutination test using reagent antibodies to human IgG and complement components. When the antiglobulin reagent is combined with washed RBCs, the Fc portion of the coating antibody molecules will bind to the reagent forming a bridge between adjacent RBCs to produce visible agglutination. Agglutination by a monospecific reagent suggests that the RBCs are coated with the corresponding antibody protein. DAT testing may be performed on automated analyzer or standard tube method using polyspecific DAT reagent. Further testing with DAT IgG specific and Complement will be performed if reactivity noted with polyspecific DAT reagent.
- Synonyms
- DAT, Direct Coombs Test
- Components
-
Code Name %DBS DAT, Broad Spectrum Coombs Serum DATCM DAT Comment
Interpretation
- Reference Range
- See individual components
Ordering & Collection
- Specimen Type
- Blood
- Collection
-
Preferred: 6 mL PINK TOP (EDTA) tube
Also acceptable: 6 mL LAVENDER TOP tube - Handling Instructions
Specimen verification and collection
- Verification of positive patient ID must be performed in the presence of the patient by the person collecting the specimen and a second qualified staff member at the time of collection and include a verbal read back of the full legal name, spelling of the legal name, and MRN as it appears on the armband.
- Both persons performing the verification are required to sign the labeled sample documenting verification was performed and the specimen was collected from the correct patient.
- Label specimen in the presence of the patient.
- Write collection date and time on specimen label.
- Signatures or initials of person collecting the specimen and second verifier are required on the specimen
Transport: Place specimen in separate biohazard bag and transport at room temperatureTransport temperature: Ambient
- Quantity
-
Requested: 6 mL EDTA whole blood
Minimum: 3 mL EDTA whole blood
Processing
- Receiving Instructions
For UWMC patients: send specimen to appropriate transfusion service lab. H location specimen will be sent to HMC, U location specimen will be sent to UWMC Montlake TSL.
For non UWMC clients: Specimen must be collected and tested at UWMC Montlake Transfusion Services Lab within 24 hours of collection. Testing will be performed at UWMC Montlake Transfusion Services. Batch and send specimen separate from other testing, indicate for “For Transfusion Services” on bag.
SPS: Receive batch from non UWMC clients and immediately send specimen to NN601, tube station: 361 for testing.
- Misc Sendout
Performance
- Lab Department
- Blood Bank(BB)
- Frequency
- Daily
- Available STAT?
- No
- Performing Location(s)
-
HMC Transfusion Service
206-520-4600325 9th Ave, Rm # BCT-67, Seattle, WA 98104-2420
UW-MT Transfusion Service
206-520-4600Clinical Lab, Room # NN601,
University of Washington Medical Center,
1959 NE Pacific street, Seattle, WA 98195
Billing & Coding
- CPT Codes
- 86880
- LOINC
- 1007-4
- Interfaced Order Code
- UOW5189