The concentrations of five vitamin D metabolites are determined by immunoaffinity extraction followed by liquid chromatography-tandem mass spectrometry. The reportable range is 5-200 pg/mL for both 1,25(OH)2D2 and 1,25(OH)2D3, 0.1 to 100.0 ng/mL for 24,25(OH)2D3, 1.0 to 200.0 ng/mL for both 25(OH)D2 and 25(OH)D3. with imprecision between 8.7 and 13.0 %CV, 5.1 and 6.0 %CV, and 4.5 and 8.0 %CV, respectively.
Code | Name |
---|---|
IVITDM | CYP24A1 Screen IA_MS Method |
125VD2 | 1,25 Dihydroxy Vitamin D2 |
125VD3 | 1,25 Dihydroxy Vitamin D3 |
125VDT | 1,25 Dihydroxy Vitamin D Total |
2425VD | Vitamin D3 (24,25_Dihydroxy) |
IVITD2 | Vitamin D2 (25_Hydroxy) |
IVITD3 | Vitamin D3 (25_Hydroxy) |
IVITDT | Vitamin D (25_Hydroxy) Total |
24DRIN | Vitamin D Ratio Information |
24DRRE | Vitamin D Ratio Result |
Serum immunoaffinity extraction - Isotope dilution liquid chromatography mass spectrometry
All Locations:
Preferred: 4 mL blood in GOLD SST tube
Also Acceptable: 4 mL blood in ORANGE RST or RED TOP tube
Pediatric: 2 Full GOLD MICROTAINER
Freeze serum at -20°C
UW-MT |
Chemistry, General
206-520-4600 Clinical Lab, Room NW220, |
---|