CYP24A1 Deficiency Screen

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General Information

Lab Name
CYP24A1 Deficiency Screen
Lab Code
CYP24A
Epic Ordering
CYP24A1 Deficiency Screen
Description

The concentrations of five vitamin D metabolites are determined by immunoaffinity extraction followed by liquid chromatography-tandem mass spectrometry. The reportable range is 5-200 pg/mL for both 1,25(OH)2D2 and 1,25(OH)2D3, 0.1 to 100.0 ng/mL for 24,25(OH)2D3, 1.0 to 200.0 ng/mL for both 25(OH)D2 and 25(OH)D3. with imprecision between 8.7 and 13.0 %CV, 5.1 and 6.0 %CV, and 4.5 and 8.0 %CV, respectively.

Synonyms
Dihydroxyvitamin D
Components

Interpretation

Method

Serum immunoaffinity extraction - Isotope dilution liquid chromatography mass spectrometry

Reference Range
See individual components
Guidelines

Ordering & Collection

Specimen Type
Serum
Collection

All Locations:

Preferred: 4 mL blood in GOLD SST tube

Also Acceptable: 4 mL blood in ORANGE RST or RED TOP tube

Pediatric: 2 Full GOLD MICROTAINER

Handling Instructions
Quantity
requested: 1 mL serum
minimum: 0.8 mL serum

Processing

Processing

Freeze serum at -20°C

Performance

LIS Dept Code
Chemistry, University (CHU)
Performing Location(s)
UW-MT Chemistry, General
206-520-4600

Clinical Lab, Room NW220,
University of Washington Medical Center,
1959 NE Pacific street, Seattle, WA 98195

Frequency
Tuesday, results next day
Available STAT?
No

Billing & Coding

CPT codes
82306, 82652, 84591
LOINC
43134-6
Interfaced Order Code
UOW3093