Direct Bilirubin (Roche)

General Information

Guidelines

Method

Spectrophotometry

Reference Range

Units: mg/dL

Female		Male		Unknown
Age	Range	Age	Range	Age	Range
0-	0.0-0.4	0-	0.0-0.4	0-	0.0-0.4

Effective date: 01/12/2026

Specimen Type: Peripheral Blood
Collection: HMC and UWMC Onsite Locations
Preferred: 4 mL blood in LIME GREEN PST tube
Also Acceptable: 4 mL blood in Orange RST, Gold SST, Red Top, and Green Top tube
Pediatric: 1 full LIME Green Microtainer
Unacceptable: Gray Top

Offsite Clinic or Other Locations
Preferred: 4 mL blood in Gold SST tube
Also Acceptable: 4 mL blood in Orange RST, Gold SST, Red Top, and Green Top tube
Pediatric: 1 full LIME Green Microtainer
Unacceptable: Gray Top
Quantity: Requested: 0.5 mL serum/plasma
Minimum: 0.1 mL serum/plasma

Receiving Instructions: Centrifuge and separate serum/plasma from cells within 2 hours. Deliver specimen directly to Lab.
Outside and Remote Locations: Spin and separate serum/plasma from cells within 2 hours. Aliquot in brown tube or wrap in foil to protect from light. Refrigerate.
Note: Direct bilirubin cannot be added on to a specimen more than 16 hours after it has already gone through the automation line and stored.

Lab Department

Chemistry, Automated Panels(CHA)

Frequency

Monday through Sunday

Available STAT?

Yes

Performing Location(s)

FHCC	Alliance Lab, Immunotherapy Lab
FHCC	FHCC Peninsula Laboratory 19917 7th Ave NE Ste 100, Poulsbo, WA 98370-6555	Phone: 360-842-5116 Fax: 360-842-5150 Hours of Operation: 8:00 a.m. - 5:00 p.m.
FHCC	Fred Hutch Alliance Lab 206-606-1088 825 Eastlake Ave, Seattle, WA 98109	Fax: 206-606-2027 Hours of Operation: 6:00 a.m. - 10:00 p.m. Includes satellite labs: • SAIM - Sloan Clinic 1, 6th Floor • FHH3 - Sloan Clinic 2, 3rd Floor • FHH4 - Sloan Clinic 2, 4th Floor
FHCC	Fred Hutch Alliance Lab 206-606-1088 825 Eastlake Ave, Seattle, WA 98109	Fax: 206-606-2027 Hours of Operation: 6:00 a.m. - 10:00 p.m. Includes satellite labs: • SAIM - Sloan Clinic 1, 6th Floor • FHH3 - Sloan Clinic 2, 3rd Floor • FHH4 - Sloan Clinic 2, 4th Floor
FHCC	Fred Hutch Community Lab, Evergreen Campus 425-441-2640 10240 NE 128th St Kirkland, WA 98034	Fax: 425-441-2707 Hours of Operation: 7:30 a.m. - 5:00 p.m.
HMC	Chemistry, Automated 206-520-4600 325 9th Ave, Rm # GWH-47, Seattle, WA 98104-2420
UW-MT	Chemistry, Automated 206-520-4600 Clinical Lab, Room NW220, University of Washington Medical Center, 1959 NE Pacific street, Seattle, WA 98195
UW-NW	Main Lab 206-668-1344 UW Medical Center – Northwest 1550 N 115th Street, A200 Seattle, WA 98133