Parvovirus B19 by PCR, Quantitative

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General Information

Lab Name
Parvo B19 by PCR, Quant
Lab Code
B19PCQ
Epic Ordering
Parvovirus B19 Quantative PCR, Blood/CSF
Description

The detection and quantitation of Parvovirus B19 by real-time PCR amplification. The primers used in this PCR reaction amplify the non-structural protein region. Quantitation of B19 (IU/mL) is achieved by amplifying standard curves consisting of serial dilutions of a known number of DNA containing the B19 amplicons. The minimum virus level that gives a positive result in 95% of replicates is 250 IU/mL (2.40 log IU/mL).

This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration.

For Tissue Biopsy, Bone Marrow, Swab, or Cellular fluid (e.g. BAL or Washing), see Parvovirus B19 by PCR Qualitative Tissue or Cells [B19QLT].

Synonyms
B19 Parvovirus, Human B19 Virus
Components

Interpretation

Method

Real-time Polymerase Chain Reaction.

Reference Range
See individual components

Ordering & Collection

Specimen Type
Blood, CSF, Clear non-cellular fluid (e.g. BAL, or Washing)
Collection

Blood: 3 mL blood in EDTA (LAVENDER TOP) tube.

CSF: 1 mL in sterile container.

Clear non-cellular fluid (e.g. BAL or Washing): 1 mL in sterile container.

Also Acceptable: 3 mL blood in GOLD SST, RED, or PPT (PEARL TOP) tube.

Unacceptable samples: Plasma collected in sodium or lithium heparin.

Note: Separate specimens MUST be submitted when both Viral Culture/Serology and PCR testing are ordered.

Minimum sample requirement: 0.5 mL

Handling Instructions
Quantity
requested: 1 mL
minimum: 0.5 mL

Processing

Processing

Blood: Separate plasma/serum from whole blood within 24 hours of collection. Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

CSF: If CSF has cellular material, centrifuge & aliquot clear CSF. Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Clear non-cellular fluid: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Outside Laboratory: Ship samples on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Shipment time must not exceed 48 hours from collection. Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Performance

LIS Dept Code
Virology, Eastlake (EVIR)
Performing Location(s)
Other Virology, Molecular
206-685-6656

Virology Laboratory, Ste 320
1616 Eastlake Ave E.
Seattle, WA 98102

Frequency
Tuesday. Results same day.
Available STAT?
No

Billing & Coding

CPT codes
87799
LOINC
26435-8
Interfaced Order Code
UOW1251