Anti Phospholipid Panel

General Information

Lab Name

Lab Code

APLSP

Epic Ordering

Anti Phospholipid Panel

Description

Synonyms

Antibodies to cardiolipin, Antiphospholipid panel, APHOSG, B2GP1, beta 2 glycoprotein 1

Components

Code	Name
AB2GR	Anti Beta2 Glycoprotein 1, IgG
AB2GC	Anti B2GP1 IgG Comment
ACLGR	Anti Cardiolipin, IgG
ACLGC	Anti Cardiolipin, IgG Comment
AB2MR	Anti Beta2 Glycoprotein 1, IgM
AB2MC	Anti B2GP1 IgM Comment
ACLMR	Anti Cardiolipin, IgM
ACLMC	Anti Cardiolipin, IgM Comment

Method

Multiplex immunoassay

Reference Range

See individual components

Guidelines

Specimen Type: Plasma or Serum
Collection: Preferred: 3 mL blood in CITRATED Blue Top. RED Top or SST Tube are also acceptable.
Handling Instructions: Refrigerate (preferred), Frozen (acceptable)
Quantity: requested: 1 mL plasma or serum
minimum: 0.8 mL

Processing: UW-MT and HMC instructions:

If Blue Top Tube AND Lupus Anticoagulant (or other coags) ordered as well: Take Blue top tube to Coag Lab for processing within 4 hours of collection. Coag tech will aliquot and refrigerate plasma sample (minimum 0.8 mL) for Immunology Lab.

If Blue Top Tube for APLSP only: Centrifuge and separate plasma within 4 hours of collection. Refrigerate plasma (only one aliquot needed).

If Red Top or SST Tube: Centrifuge and separate serum within 2 hours of collection. Refrigerate serum (only one aliquot needed).

Outside Laboratory:

Blue Top Tube: Centrifuge Blue top for 10 minutes, remove plasma and re-spin plasma for another 10 minutes. Decant and Freeze plasma (minimum 0.8 mL) at -20°C to -80°C. Send Frozen on dry ice.

Red Top or SST Tube: Centrifuge and separate serum within 2 hours of collection. Refrigerate serum (minimum 0.8 mL).

Stability: Refrigerate up to 14 days, freeze for longer storage. Avoid repeated freeze/thaw cycles.

LIS Dept Code

Immunology (IMM)

Performing Location(s)

UW-MT	Immunology 206-520-4600 Clinical Lab, Room NW220, University of Washington Medical Center, 1959 NE Pacific street, Seattle, WA 98195

Frequency

M-F

Available STAT?