Activated Protein C Resistance
General Information
- Lab Name
- Activated Protein C Resistance
- Lab Code
- APCR
- Epic Ordering
- Activated Protein C Resist.
- Description
The Activated Protein C Resistance (APCR) is a plasma based functional assay used to screen for the factor V Leiden mutation (F5:p.R506Q). Patients with factor V Leiden mutation have increased risk for incidental or recurrent venous thromboembolism.
- Synonyms
- APC Resistance, Factor 5 Leiden Protein Assay, Factor V Leiden Protein Assay, Protein C Cofactor
Interpretation
- Method
Clot based
- Reference Range
-
Female Male Age Range Age Range 0- Negative [NRN] 0- Negative [NRN] Effective date: 04/01/2010
- Interferences and Limitations
Patients on Aprotinin, Protamine or direct thrombin inhibitors such as hirudin (Refludan), bivalidrudin (Angiomax), Dabigatran (Pradaxa), and argatroban (Novastan) may interfere with this assay. If patients were administered with Aprotinin, Protamine or direct thrombin inhibitors, it is recommended to wait at least 24 hours before collecting samples or order F5DNA assay.
Ordering & Collection
- Specimen Type
- Blood
- Collection
-
3 or 5 mL BLUE (CITRATE) tube
- Forms & Requisitions
Outside clients should fill and submit Coagulation Patient Clinical History Form: Coagulation Patient Clinical History Form
- Approval Required
- **Laboratory Medicine resident's approval is required for hospital inpatients and patients in Emergency Department.**
- Handling Instructions
The Laboratory MUST process specimen within 4 hours of blood collection.
- Quantity
-
requested: entire sample
minimum: 1.0 mL plasma
Processing
- Processing
**Laboratory Medicine resident's approval is required for hospital inpatients and patients in Emergency Department.** Approval is NOT required for hospital outpatients, clinic patients or outside clients.
UW-MT Instructions: Take specimen to UW-MT Coag for processing. Coag tech will freeze plasma sample for transport to HMC Coag."
HMC Instructions: Take specimen to HMC Coag bench for processing.
Outside Laboratory:Centrifuge for 10 minutes, remove plasma & re-spin plasma for another 10 minutes. Decant & Freeze plasma (minimum 1.0 mL) @ -20°C to -80°C. Send Frozen on dry ice.
Performance
- LIS Dept Code
- Coagulation (COAG)
- Performing Location(s)
-
HMC Coagulation
206-520-4600325 9th Ave, Rm # GWH-47, Seattle, WA 98104-2420
- Frequency
- Run Monday and Thursday. Reported by end of day.
- Available STAT?
- No
Billing & Coding
- CPT codes
- 85307
- LOINC
- 52750-7
- Interfaced Order Code
- UOW885
- Interfaced Result Code
- UOW885