Activated Protein C Resistance

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General Information

Lab Name
Activated Protein C Resistance
Lab Code
APCR
Epic Ordering
Activated Protein C Resist.
Description

The Activated Protein C Resistance (APCR) is a plasma based functional assay used to screen for the factor V Leiden mutation (F5:p.R506Q). Patients with factor V Leiden mutation have increased risk for incidental or recurrent venous thromboembolism.

Forms & Requisitions

Outside clients should fill and submit Coagulation Patient Clinical History Form: Coagulation Patient Clinical History Form

Synonyms
APC Resistance, Factor 5 Leiden Protein Assay, Factor V Leiden Protein Assay, Protein C Cofactor

Interpretation

Method

Clot based

Reference Range
Female Male
AgeRangeAgeRange
0-Negative [NRN] 0-Negative [NRN]

Effective date: 04/01/2010

Interferences and Limitations

Patients on Aprotinin, Protamine or direct thrombin inhibitors such as hirudin (Refludan), bivalidrudin (Angiomax), Dabigatran (Pradaxa), and argatroban (Novastan) may interfere with this assay. If patients were administered with Aprotinin, Protamine or direct thrombin inhibitors, it is recommended to wait at least 24 hours before collecting samples or order F5DNA assay.

Ordering & Collection

Specimen Type
Blood
Collection

3 or 5 mL BLUE (CITRATE) tube

Forms & Requisitions

Outside clients should fill and submit Coagulation Patient Clinical History Form: Coagulation Patient Clinical History Form

Approval Required
**Laboratory Medicine resident's approval is required for hospital inpatients and patients in Emergency Department.**
Handling Instructions

The Laboratory MUST process specimen within 4 hours of blood collection.

Quantity
requested: entire sample
minimum: 1.0 mL plasma

Processing

Processing

**Laboratory Medicine resident's approval is required for hospital inpatients and patients in Emergency Department.** Approval is NOT required for hospital outpatients, clinic patients or outside clients.

UW-MT Instructions: Take specimen to UW-MT Coag for processing. Coag tech will freeze plasma sample for transport to HMC Coag."

HMC Instructions: Take specimen to HMC Coag bench for processing.

Outside Laboratory:Centrifuge for 10 minutes, remove plasma & re-spin plasma for another 10 minutes. Decant & Freeze plasma (minimum 1.0 mL) @ -20°C to -80°C. Send Frozen on dry ice.

Performance

LIS Dept Code
Coagulation (COAG)
Performing Location(s)
HMC Coagulation
206-520-4600

325 9th Ave, Rm # GWH-47, Seattle, WA 98104-2420

Frequency
Run Monday and Thursday. Reported by end of day.
Available STAT?
No

Billing & Coding

CPT codes
85307
LOINC
52750-7
Interfaced Order Code
UOW885
Interfaced Result Code
UOW885