Mumps PCR to Washington State Lab (Sendout)
General Information
- Lab Name
- Lab Code
- 643
- Description
Useful For: This test is used for suspected cases of acute disease.
Ordering Requirements:
- Prior to sample submission, providers must obtain approval by contacting the Seattle King Co. Public Health Epidemiologist at (206) 296-4774. If patient is a resident of another county, contact the appropriate LHJ for permission (see resource below for contact information). LHJ staff must then notify Washington State Department of Health (DOH) Office of Communicable Disease Epidemiology (CDE) at 206-418-5500.
- WA State Interactive Map of LHJ Websites
- Prior to sample submission, providers must obtain approval by contacting the Seattle King Co. Public Health Epidemiologist at (206) 296-4774. If patient is a resident of another county, contact the appropriate LHJ for permission (see resource below for contact information). LHJ staff must then notify Washington State Department of Health (DOH) Office of Communicable Disease Epidemiology (CDE) at 206-418-5500.
- Components
Interpretation
- Guidelines
- Method
Real-Time Reverse Transcriptase Polymerase Chain Reaction (rRT-PCR)
- Reference Range
Ordering & Collection
- Specimen Type
- Buccal Swab, Urine
- Collection
-
Buccal Swab: Collect buccal swab specimen and place in viral transport media tube (2-3 mL VTM). Use only synthetic tip swabs (dacron, nylon, polyester) with non-wooden shaft.
- For optimal isolation, collect within 72 hours of symptom onset.
Urine: Collect 50 ml urine in sterile screw-capped container.
- Collect 4-10 days post symptom onset.
Washington State Department of Health Specimen Collection and Submission Instructions for Mumps RT-PCR.
- Handling Instructions
Outside Laboratories (Washington State Only): Providers must obtain permission from the local county public health epidemiologist prior to submitting a sample. If sender is unable to complete an electronic order in the WA DOH ETOR portal, the following information is required: ordering provider name and NPI, patient full name, dob, sex, and address.
- Quantity
-
Requested: Buccal Swab in 2-3 mL VTM or 50 mL Urine
Minimum: Buccal swab in 2 mL VTM or 20 mL Urine
Processing
- Receiving Instructions
Refrigerate buccal swab/VTM and urine specimens. Refrigerated samples must be received by WPHL within 72 hrs. Freeze at ≤-70°C if samples are being stored for >24 hours.
Login: SEND1-;REFRIG
- RSNDT1: WPHL
- RSTYP1: BUCSWB [and/or] URN
- RTSRQ1: ;Mumps by PCR
Sendouts:
- Place the order in the WA DOH ETOR (Electronic Test Ordering & Results Portal).
- Manual requisitions are no longer accepted by WAPHL.
- Order Test: Mumps, RT-PCR under General Microbiology/Virology. Enter a separate order for each sample type received.
- Print the resulting requisition and package it with the sample.
- Send samples to WPHL via DLMP or Delivery Express courier.
Stability: Refrigerated: 72 hours; Frozen (-70°C): Samples >72 hours old; Ambient: Unacceptable.
- Misc Sendout
Performance
- Lab Department
- Frequency
- Performed within 24 hours of sample arrival. Turnaround Time: 3 days.
- Available STAT?
- Yes, by arrangement with Department of Health Communicable Disease Epidemiology.
- Performing Location(s)
-
Sendout Washington State Public Health Laboratory
206-418-54001610 NE 150th St.
Shoreline, WA 98155