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Ebola Virus PCR to Washington State Lab (Sendout)

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General Information

Lab Name
Lab Code
595
Description

Ordering Requirements:

  • Pre-approval required. Providers must consult the Local Health Jurisdiction (LHJ) epidemiologist of the patient's county of residence and the Washington State CDE.
    • Seattle King Co. Public Health Epidemiologist: 206-296-4774
    • WA DOH Office of Communicable Disease Epidemiology (CDE): 206-418-5500
Forms & Requisitions

Synonyms
Ebola Hemorragic Fever, EVD
Components

Interpretation

Guidelines
Method

Real-Time Reverse Transcriptase Polymerase Chain Reaction (rRT-PCR)

Reference Range

Ordering & Collection

Specimen Type
Blood
Collection

Collect 2 LAVENDER TOP (EDTA) tubes with 5 mL blood in each.

  • Note that this test requires dedicated tubes; samples cannot be shared.

Unacceptable: GREEN TOP (Heparinized) tubes or samples collected in glass tubes.

Expedite transport to the lab.

WA DOH Specimen Collection and Submission Instructions: Ebola Virus

Forms & Requisitions

Handling Instructions

Outside Laboratories: Test not available through UW DLMP. Arrange for testing directly with WA DOH or the Local Health Jurisdiction.

Quantity
Requested: 2 x 5 mL whole blood EDTA tubes
Minimum: 2 x 2 mL whole blood EDTA

Processing

Receiving Instructions

Place unspun whole blood samples in a separate biohazard ziplock bag with absorbent. Store refrigerated.

SPS Note: At order entry, include the comment "POSSIBLE EBOLA" in the modifier for all tests requested.

Login: SPMSND-;REFRIG, POSSIBLE EBOLA

  • MSNDTO: WPHL
  • MSPTYP: WB
  • MTSTRQ: ;Ebola Virus by PCR

Sendouts:

  • Prior to sending, confirm approval with the SKPH Epidemiologist at (206) 296-4774 or WPHL Epidemiologist at (206) 418-5500.
  • Place the order in the WA DOH ETOR (Electronic Test Ordering & Results Portal).
    • Manual requisitions are no longer accepted by WAPHL.
  • Order Test: Rule out Ebola Virus under "WA General Microbiology: Bioterrorism."
  • Include a note in the order to “page Lab Medicine Resident on-call with results at 206-598-6190."
  • Forward samples and electronic requisition to UW-MT or HMC Microbiology for packaging/transport (UW-NW: May be packaged by trained SPS staff.)

Transport: Samples must be packaged and transported as Category A (UN 2814 Infectious Substance, Affecting Humans) according to USDOT and IATA regulations by trained staff only. Send samples refrigerated with a copy of the WPHL electronic requisition.

Delivery Address:
Washington State Public Health Laboratories
Attn: Bioterrorism Lab
1610 NE 150th St, Shoreline, WA 98155

Stability: Refrigerated (preferred): 48 hours.

Misc Sendout

Performance

Lab Department
Frequency
Performed: As needed. Turnaround Time: 8 days.
Available STAT?
Yes, by arrangement with WA State DOH.
Performing Location(s)
Sendout Washington State Public Health Laboratory
206-418-5400

1610 NE 150th St.
Shoreline, WA 98155

Billing & Coding