Neoplasia G-banded Chromosome Analysis and Karyotyping [UWMC Cytogenetics and Genomics]

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General Information

Lab Name
Lab Code
543
Description

This test detects acquired chromosomal aberrations, including both balanced (translocations and inversions) and imbalanced (aneuploidy, deletions, duplications, or amplification) rearrangements in the genome at low resolution. Testing is performed by UWMC - Montlake DLMP Cytogenetics and Genomics Laboratories.

EPIC Ordering:

  • Test Name: Karyotype, Neoplasia
  • Order Code: KARYONEOP
  • For orders submitted electronically via Epic, a separate requisition form is not required.
Forms & Requisitions

Cytogenetics Neoplasia Test Request Form

Synonyms
Acute Promyelocytic Leukemia, Amyloidosis, APL, B-Cell Lymphoma, Blast Crisis, Bleeding Disorder, Burkitt's Lymphoma, Carcinoma, Diffuse Large B-Cell Lymphoma, DLBCL, Elevated Platelets, Ewing Sarcoma, Fanconi Anemia, Follicular Lymphoma, Hairy Cell Leukemia, Hemangioma, Hodgkin Lymphoma, Idiopathic Thrombocytopenic Purpura, Inversion, KARYONEOP, Karyotype, Leukemia, Lipoma, Liposarcoma, Lymphoplasmacytic Leukemia, Lymphoproliferative Disorder, Macroglobulinemia, MALT Lymphoma, Mantle Cell Lymphoma, Marginal zone Lymphoma, Mastocytosis, MDS, Melanoma, MGUS, MM, Monoclonal Gammopathy, Monosomy, MPD, Multiple Myeloma, Mycosis Fungoides, Myelodysplastic Syndrome, Myeloproliferative Disorder, Neuroblastoma, NHL, Non-Hodgkin Lymphoma, Pancytopenia, Plasmacytoma, Polycythemia, Promyelocytic Leukemia, Refractory Anemia, Rhabdomyosarcoma, Synovial Sarcoma, T-Cell Leukemia, T-Cell Lymphoma, Treatment-Related Leukemia, Waldenstrom's Macroglobulinemia
Components

Interpretation

Reference Range
Interferences and Limitations

The standard cytogenetic methodology used in this analysis does not routinely detect subtle submicroscopic balanced rearrangements, abnormalities present at a low level (screening of additional cells can be ordered if this is suspected), or rearrangements below the resolution of the banding level.

Ordering & Collection

Specimen Type
Bone Core, Bone Marrow, Cerebrospinal Fluid, Leukemic Peripheral Blood, Lymph Node, Paracentesis Fluid, Solid Tumor
Collection

Bone Marrow:

  • Collect up to 5 mL in preservative-free sodium heparin (green top vacutainer) OR in a sterile heparinized syringe.

Bone Core or Lymph Node:

  • Immediately place in a sterile vial with 3-5 mL tissue transport media (supplied by the lab - call 206.598.4488).

Leukemic Peripheral Blood:

  • Collect 10 mL of blood in preservative-free sodium heparin (green top vacutainer) OR in a sterile heparinized syringe.

Paracentesis Fluid or Cerebrospinal Fluid:

  • Collect in a sterile vial.

Solid Tumor:

  • Obtain sample (0.15-2.0cm3) under sterile conditions and place in transport media (supplied by the lab - call 206.598.4488). Keep refrigerated.
Forms & Requisitions

Cytogenetics Neoplasia Test Request Form

Handling Instructions

Outside Laboratories:

  • Maintain and transport samples at ambient temperature unless otherwise indicated in the Collection section.
  • A completed request form must accompany all orders.
  • Specimens are accepted 24 hrs/day, 7 days/week.
  • Ship to:
    • Cytogenetics and Genomics Laboratory
      University of Washington Medical Center, Room NW-125
      1959 NE Pacific Street
      Seattle, WA 98195-6100
Quantity
Requested: Varies; refer to Collection information.
Minimum: Varies; refer to Collection information.

Processing

Receiving Instructions

Hold specimen(s) at room temperature.

  • Call Cytogenetics and Genomics Laboratory at 206.598.4489 for pick up. Cytogenetics is staffed on weekends to process specimens.
  • If no answer or after hours, call 206.598.4488 to leave message.
Misc Sendout

Performance

Lab Department
Frequency
Performed daily. TAT for bone marrow, bone core, leukemic blood, or lymph node: 5-7 days. TAT for solid tumor: 14-21 days.
Available STAT?
Yes
Performing Location(s)
Pathology Cytogenetics Laboratory
206-598-4488
Other Locations/Notes

Cytogenetics and Genomics Laboratory

University of Washington Medical Center, Room NW-125
1959 NE Pacific Street
Seattle, WA 98195-6100

  • Ph: (206) 598-4488
  • Fax: (206) 598-2610
  • Email: cytogene@uw.edu

Billing & Coding

Billing Comments

CPTs:

  • Bone Marrow, Bone Core, Leukemic Peripheral Blood, or Lymph Node: 88237, 88264, 88291
  • Solid Tumor: 88239, 88264, 88291