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Neoplasia IFISH [UWMC Cytogenetics and Genomics]

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General Information

Lab Name
Lab Code
423
Description

This test uses a variety of FISH probes to detect specific rearrangements associated with neoplastic disorders, including both balanced (translocations and inversions) and imbalanced (aneuploidy, deletions, duplications, or amplification) rearrangements. Testing is performed by UWMC - Montlake DLMP Cytogenetics and Genomics Laboratories.

EPIC Ordering:

  • Test Name: FISH, Neoplasia
  • Order Code: LAB015
  • For orders submitted electronically via Epic, a separate requisition form is not required.
Synonyms
13q deletion, 20q deletion, 4q12 deletion, 4q12 rearrangement, 5q deletion, 5q33 rearrangement, 7q deletion, ABL1, Acute Lymphocytic Leukemia, Acute Myeloid Leukemia, Acute Promyelocytic Leukemia, ALK, ALL, AML, Amyloidosis, Anemia, APL, ASS1, ATM, B-cell lymphoma, BCL2, BCL6, BCR, BCR/ABL1, BIRC3, Blast Crisis, Bleeding Disorder, Burkitt's Lymphoma, Carcinoma, CBFB, CCND1, CDKN2A, CEN12, CEN15, CEN17, CEN7, CEN8, CEN9, CHIC2, Chronic Lymphocytic Leukemia, Chronic Myelogenous Leukemia, Chronic Myelomonocytic Leukemia, CLL, CML, CMML, D13S319, D20S108, D5S23, D5S721, D7S486, Diffuse Large B-Cell Lymphoma, EGFR, EGR1, Elevated Platelets, Eosinophilia, ETV6, EVI1, EWSR1, Fanconi Anemia, FGFR1, FGFR3, FIP1L1, FISH, Fluorescence In Situ Hybridization, Follicular Lymphoma, FOXO1, Hairy Cell Leukemia, Hemangioma, Hemolysis, HES, Hodgkin Lymphoma, Hypereosinophilic syndrome, Idiopathic Thrombocytopenic Purpura, IGH, inv(16), inv(3), KIT, LAB015, Leukemia, Lipoma, Liposarcoma, LNX, Lymphoma, Lymphoproliferative Disorder, Macrocytoma, Macroglobulinemia, MAF, MALT, MALT1, Mantle Cell Lymphoma, Marginal Cell Lymphoma, Mastocytosis, MDS, Melanoma, MLL, MM, Monoclonal Gammopathy, Monosomy, MPD, Multiple Myeloma, MYB, MYC, Mycosis Fungoides, Myelodysplastic Syndrome, Myeloma, Myeloproliferative Neoplasm, Neoplasia, Neuroblastoma, Night Sweats, Non-Hodgkin Lymphoma, Pancytopenia, PDGFRA, PDGFRB, Plasmacytoma, PML, Polycythemia, RARA, Refractory Anemia, ROS1, RUNX1, RUNX1T1, Sarcoma, SCFD2, SS18, Systemic Mast Cell Disease, t(11;14), t(14;18), t(15;17), t(8;21), t(9;22), T-Cell Leukemia, T-Cell Lymphoma, TCL1A, THOR, Thrombocytopenia, TP53, TRA, TRB, TRD, Treatment-Related Leukemia, Waldenstrom's Macroglobulinemia
Components

Interpretation

Guidelines
Reference Range

Ordering & Collection

Specimen Type
Bone Core, Bone Marrow, Cerebral Spinal Fluid, FFPE Tissue, Formalin Fixed Paraffin Embedded Tissue, Leukemic Peripheral Blood, Lymph Node, Paracentesis Fluid, Solid Tumor
Collection

Bone marrow:

  • Collect up to 5 mL in preservative-free sodium heparin (green top vacutainer) OR in a sterile heparinized syringe.

Bone core or lymph node:

  • Immediately place in a sterile vial with 3-5 mL tissue transport media (supplied by the lab - call 206.598.4488).

Leukemic Peripheral Blood:

  • Collect 10 mL of blood in preservative-free sodium heparin (green top vacutainer) OR in a sterile heparinized syringe.

Paracentesis Fluid or Cerebrospinal Fluid:

  • Collect in a sterile vial.

Solid Tumor (fresh):

  • Obtain sample (0.15-2.0cm3) under sterile conditions and place in transport media (supplied by the lab - call 206.598.4488).
  • Keep refrigerated.

Solid Tumor (FFPE/Formalin-Fixed, Paraffin-Embedded):

  • Provide an unstained slide (4 μm thick section) per probe of IFISH and a corresponding H&E stained slide.
  • Do not send specimens that have been exposed to decalcifying agents.
  • Mail slides in a protected mailing container.
Forms & Requisitions
Handling Instructions

Outside Laboratories:

  • Maintain and transport samples at ambient temperature unless otherwise indicated in the Collection section.
  • A completed request form must accompany all orders.
  • Specimens are accepted 24 hrs/day, 7 days/week.
  • Ship to:
    • Cytogenetics and Genomics Laboratory
      University of Washington Medical Center, Room NW-125
      1959 NE Pacific Street
      Seattle, WA 98195-6100
Quantity
Requested: Varies; refer to Collection information.
Minimum: Varies; refer to Collection information.

Processing

Receiving Instructions

Hold specimen(s) at room temperature.

  • Call Cytogenetics and Genomics Laboratory at 206.598.4489 for pick up. Cytogenetics is staffed on weekends to process specimens.
  • If no answer or after hours, call 206.598.4488 to leave message.
Misc Sendout

Performance

Lab Department
Frequency
Performed daily. TAT for fresh samples: 1-6 days. TAT for FFPE samples: 2-8 days.
Available STAT?
Yes
Performing Location(s)
Pathology Cytogenetics Laboratory
206-598-4488
Other Locations/Notes

Cytogenetics and Genomics Laboratory

University of Washington Medical Center, Room NW-125
1959 NE Pacific Street
Seattle, WA 98195-6100

  • Ph: (206) 598-4488
  • Fax: (206) 598-2610
  • Email: cytogene@uw.edu

Billing & Coding

Billing Comments

CPTs:

  • Fresh samples: 88237, 88271, 88275 (per 200 cells), 88291
  • FFPE samples: 88271, 88275 (per 200 cells), 88291