Borrelia Species by PCR (Sendout)

General Information

Lab Name
Lab Code: 260
External Test Id: 0055570
Description: Ordering Recommendation: Not a first-line test for Lyme disease. May be useful if strong suspicion of Lyme disease persists in spite of persistent negative serologic testing. Blood and CSF specimens have poor clinical sensitivity for detection of Borrelia burgdorferi by PCR.

For Lyme Disease Serology testing, refer to Lyme Disease Serology w/ Conf (Sendout) [RLYMES].
Synonyms: 0055570, B. burgdorferi DNA detection, Borrelia burgdorferi, Lyme Disease, Lyme Disease PCR, Tick Borne Disease

Method

Qualitative Polymerase Chain Reaction

Ref. Range Notes

Reference Range: Not Detected

Guidelines

Specimen Type

Blood, CSF, Tissue, Synovial Fluid

Collection

Blood: collect 5 mL in LAVENDER TOP (EDTA) tube or GOLD SST

CSF or Synovial fluid: 1 mL fluid in a sterile container

Tissue: Transfer tissue to a sterile container

Handling Instructions

Outside Laboratories:

Blood: Centrifuge sample and transfer serum or plasma to a separate sterile aliquot. Freeze at -20°C while awaiting shipment.
CSF or Synovial Fluid: Transfer 1 mL CSF to a sterile aliquot. Freeze at -20°C while awaiting shipment.
Tissue: Transfer tissue to a sterile container and freeze at -20°C immediately.

Transport samples on dry ice. Indicate the specimen type on the UW requisition.

Stability:

Serum/Plasma/CSF/Synovial Fluid: Frozen (preferred): 1 year; Refrigerated: 72 hours; Ambient: 8 hours.
Tissue: Frozen (required): 1 year; Refrigerated: Unacceptable; Ambient: Unacceptable.

Unacceptable Conditions: Heparinized specimens, tissues in optimal cutting temperature compound.

Quantity

requested: 1 mL CSF/Synovial Fluid/Serum/Plasma
minimum: 0.5 mL CSF/Synovial Fluid/Serum/Plasma

Processing

Processing:

Blood: Centrifuge sample and transfer serum or plasma to a separate sterile aliquot. Freeze at -20°C.
CSF or Synovial Fluid: Transfer 1 mL CSF to a sterile aliquot. Freeze at -20°C.
Tissue: Transfer to a sterile container and freeze at -20°C immediately.

Login: SPMSND-;FREEZE

Specimen Type Codes: SRM, PLS, CSF, SYN, TIS (serum, plasma, cerebrospinal fluid, sunovial fluid, tissue).

Sendouts:

Stability:

Serum/Plasma/CSF/Synovial Fluid: Frozen (preferred): 1 year; Refrigerated: 72 hours; Ambient: 8 hours.
Tissue: Frozen (required): 1 year; Refrigerated: Unacceptable; Ambient: Unacceptable.

Unacceptable Conditions: Heparinized specimens, tissues in optimal cutting temperature compound.

LIS Dept Code

Performing Location(s)

Sendout	ARUP Laboratories, Inc. 800-522-2787 500 Chipeta Way Salt Lake City, UT 84108

Frequency

Performed: Monday, Wednesday, Friday. Reported: 1-4 days.

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