Ethyl Glucuronide Screen with Reflex, Urine (Sendout)

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General Information

Lab Name
Lab Code
205
External Test Id
ETGR
Description

Useful For: Screening and confirmation for drug abuse involving alcohol.

Test Algorithm: Testing begins with a screening assay. If the screen is positive, then the liquid chromatography-tandem mass spectrometry confirmation with quantification will be performed at an additional charge.

References
  1. Mayo Clinical & Interpretive Information: Ethyl Glucuronide Screen with Reflex, Random, Urine
  2. Dahl H, Stephanson N, Beck O, Helander A: Comparison of urinary excretion characteristics of ethanol and ethyl glucuronide. J Anal Toxicol. 2002;26:201-104. doi: 10.1093/jat/26.4.201
Synonyms
Alcohol biomarkers, ETGC, ETGR, ethyl sulfate

Interpretation

Method

Screen: Immunoassay
Confirmation: Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Ref. Range Notes
Reference Value: Negative
Screening cutoff concentration: 500 mg/mL

Interpretation:

If the screen result is negative, ethyl glucuronide concentrations were not detected.

If the screen result is positive, then confirmation by liquid chromatography tandem mass spectrometry will be performed.

A positive interpretation will be given if either the ethyl glucuronide (EtG) result is greater than or equal to 250 ng/mL and/or the ethyl sulfate (EtS) is greater than or equal to 100 ng/mL.

A "high" positive (ie, >1000 ng/mL) may indicate:

  • Heavy drinking on the same day or previously (ie, previous day or 2).
  • Light drinking the same day

A "low" positive (ie, 500-1000 ng/mL) may indicate:

  • Previous heavy drinking (ie, previous 1-3 days).
  • Recent light drinking (ie, past 24 hours).
  • Recent intense "extraneous" exposure (ie, within 24 hours or less).

A "very low" positive (ie, 100-500 ng/mL) may indicate:

  • Previous heavy drinking (ie, 1-3 days)
  • Previous light drinking (ie, 12-36 hours).
  • Recent "extraneous" exposure.²
Interferences and Limitations

Cautions: Care should be taken when interpreting results since there are many factors (eg, fluid intake and other biologic factors) that may influence a urine test result. It is possible that substances other than those investigated in the specificity study may interfere with the test and cause false-positive or negative results.

References
  1. Mayo Clinical & Interpretive Information: Ethyl Glucuronide Screen with Reflex, Random, Urine
  2. Dahl H, Stephanson N, Beck O, Helander A: Comparison of urinary excretion characteristics of ethanol and ethyl glucuronide. J Anal Toxicol. 2002;26:201-104. doi: 10.1093/jat/26.4.201

Ordering & Collection

Specimen Type
Urine
Collection

Collect Random Urine in a preservative-free urine collection container.

Handling Instructions

Outside Laboratories: Aliquot 2.5 mL to 5 mL urine into a separate plastic vial. Refrigerate urine.

Stability: Refrigerated (preferred): 28 days; Frozen: 28 days; Ambient: 72 hours.

Quantity
requested: 5 mL urine
minimum: 2.5 mL urine

Processing

Processing

Aliquot 2.5 to 5 mL urine into a separate screw-cap vial. Refrigerate urine.

Login: SEND1-;REFRIG

  • RSNDT1: MAYOSD
  • RSTYP1: URN
  • RTSRQ1: ;Ethyl Glucuronide Screen w/ Reflex (Mayo Test ETGR)

Sendouts:

  • Order Mayo code: ETGR

Stability: Refrigerated (preferred): 28 days; Frozen: 28 days; Ambient: 72 hours.

Performance

LIS Dept Code
Performing Location(s)
Sendout Mayo Clinic Laboratories (Superior Drive)
800-533-1710

3050 Superior Drive NW
Rochester, MN 55901
Frequency
Performed: Monday through Saturday. Report Available: 1-2 days from sample receipt at Mayo Clinic Labs.
Available STAT?
No

Billing & Coding

CPT codes
Billing Comments

CPT (Screen): 80307; CPT (Confirmation, if performed): G0480

LOINC