Chagas Disease Molecular Detection (Sendout)

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General Information

Lab Name
Lab Code
1061
External Test Id
CDC-10475
Description

Test Information: This assay is used in clinically indicated situations such as acute or congenital infections, post-transplant from a donor with confirmed T. cruzi infection, or risk of reactivation due to immunocompromise in patients with chronic Chagas disease. Serological testing is the preferred method to diagnose chronic infection in patients.

Ordering Requirements: A completed Washington State PHL Serology Requisition (or CDC 50.34 Specimen Submission Form, if sent directly) must accompany all submissions. Providers should coordinate with the LHJ epidemiologists or with the appropriate CDC lab contacts (if sending direct).

Approval: Lab Medicine Resident or Microbiology Fellow approval required. Testing must be coordinated with LHJ epidemiologists or directly with the CDC.

***Ordering Alert: Effective 12/09/2024, this test is currently unavailable at the CDC. If testing is requested, contact the Laboratory Medicine Resident or Microbiology Fellow on-call to discuss alternative testing options.***

Forms & Requisitions
Synonyms
American trypanosomiasis, CDC-10475, Chagas PCR, Trypanosoma cruzi

Interpretation

Method

Real-time Polymerase Chain Reaction (PCR)

Guidelines

Ordering & Collection

Specimen Type
Blood
Collection

Collect 5 mL blood in LAVENDER TOP (EDTA) tube

Also Accepted: PINK EDTA

Forms & Requisitions
Approval Required
Lab Medicine Resident or Microbiology Fellow approval required. Testing must be coordinated with state lab epidemiologists or pre-approved by CDC.
Handling Instructions
Quantity
requested: 5 mL EDTA whole blood
minimum: 2.2 mL EDTA whole blood (Infants <1 year old: 0.3 mL whole blood)

Processing

Processing

Refrigerate whole blood in original collection container.

Login: SPMSND-;REFRIG

  • MSNDTO: CDC
  • MSPTYP: WB
  • MTSTRQ: ;Chagas Disease Molecular Detection (CDC Test 10475)

Sendouts: Samples may be sent to WPHL or directly to the CDC, depending on instruction from the LMR/Micro Fellow.

To CDC via Washington State Public Health Lab:

  • Provider must complete the WPHL Serology Requisition. Add the CAST fax number to the form to ensure proper result routing.
  • Send sample with the DLMP courier or Delivery Express courier.

Direct Submission to CDC:

  • CDC Test: CDC-10475.
  • To prevent sample rejections, review the CDC requisition for completion prior to shipment.
    • There must be two matching patient identifiers present on the sample and on the CDC requisition. One identifier must be the name, the other identifier can be the MRN, specimen ID, or date of birth. Note that because Sunquest CID labels do not contain the DOB, then either or the MRN or specimen ID must be included on the requisition or the date of birth added to the specimen label.
    • Requisitions that are pre-filled by the provider may require additional information to be added after the sample is collected. This may include: collection date, sample type, date sent to CDC, specimen ID number, etc. Per CDC requirements, additional information may be written on an electronically completed form as long as it is initialed by lab staff making the correction/addition.
    • CAST must be entered as the "intermediate submitter" to ensure that result reports are properly routed to the lab from the WAPHL.
  • Send sample refrigerated (2-8°C) Monday - Thursday only via FedEx Priority Overnight.
    • Samples must be received within 7 days of collection.

Stability: Refrigerated (required): 7 days; Frozen: Unacceptable; Ambient: Unacceptable.

Performance

LIS Dept Code
Performing Location(s)
Sendout Centers for Disease Control and Prevention
855-612-7575

1600 Clifton Road NE
Atlanta, GA 30333
Frequency
TAT: 2 weeks.
Available STAT?
No

Billing & Coding

CPT codes
Billing Comments

No charge for CDC testing.

LOINC