JC Virus by PCR Quantitative, Urine

General Information

Lab Name

JC Virus Quantitative PCR, Urine

Lab Code

UJCVQN

Epic Name

JCV Quantitative PCR, Urine

Description

The detection and quantification of JC Virus (JCV) by real-time PCR amplification. The primers used in this PCR reaction amplify the Large T antigen gene. The JC Virus probes are labeled with reporter dye FAM and quencher TAMRA. Quantification of JC Virus DNA (IU/mL) is achieved by amplifying standard curves consisting of serial dilutions of a known number of DNA containing the JC Virus amplicons. The limit of detection (LoD), defined as the minimum viral level detectable in 95% of replicates, and the lower limit of quantification (LLoQ) are both 3000 IU/mL (3.48 log IU/mL).

This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration.

For Blood, CSF, or Clear non-cellular fluid (ex: BAL, or Washing), refer to JC Virus by PCR Quantitative [JCVQN].

For tissue biopsy, bone marrow, or cellular/viscous fluids (e.g., BAL or washings), refer to JC Virus Qualitative PCR, Tissue [JCVQLT].

Synonyms

JC Virus, PML Virus, Progressive Multifocal Leukoencephalopathy virus

Components

Code	Name
UJCVQR	JC Virus Quantitative PCR Urine Result
UJCLOG	JC Virus Quantitative PCR Urine Result (Log 10)
UJCVQI	JC Virus Quantitative PCR Urine Interpretation