JC Virus by PCR Quantitative, Urine

The detection and quantification of JC Virus (JCV) by real-time PCR amplification. The primers used in this PCR reaction amplify the Large T antigen gene. The JC Virus probes are labeled with reporter dye FAM and quencher TAMRA. Quantification of JC Virus DNA (copies/mL) is achieved by amplifying standard curves consisting of serial dilutions of a known number of DNA containing the JC Virus amplicons. The minimum virus level that gives a positive result in 95% of replicates is 500 copies/mL (2.70 log copies/mL).

This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration.

For Blood, CSF, or Clear non-cellular fluid (ex: BAL, or Washing), see JC Virus by PCR Quantitative [JCVQN].

For Tissue Biopsy, Bone Marrow, Swab, or Cellular fluid (ex: BAL or Washing), see JC Virus Qualitative PCR, Tissue [JCVQLT].