FHCC HLA typing for potential HSCT recipient and donor(s)

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General Information

Lab Name
HLA Typing HSCT, FHCC
Lab Code
SHLA
Epic Name
HLA Typing HCT, FHCC
Description

Test Information: Placeholder for samples sent to Fred Hutch Clinical Immunogenetics Laboratory (CIL). CIL provides HLA typing, crossmatching, and PRA studies for potential stem cell transplant recipients and donors.

Ordering Note: Bloodworks Northwest Immunogenetics/HLA Lab is the preferred performing lab for HLA typing and PRA studies for identifying community platelet donors.

Forms & Requisitions
  • FHCC Clinical Immunogenetics Laboratory HLA Requisition
Synonyms
HLA for Bone Marrow Transplant, HLA typing of potential stem cell transplant recipients or potential stem cell donors, PRA, SHLA
Components
Code Name
SPHLA Collection Status , HLA (HSCT)
CSHLA Collected Specimen, HLA (HSCT)

Interpretation

Method

Molecular HLA typing, Solid phase PRAs, Lymphocytotoxic and flow cytometric crossmatches

Reference Range
See individual components

Ordering & Collection

Specimen Type
Blood, Buccal swab, Saliva
Collection

Collect: 10 mL blood in RED TOP and 30 mL blood in GREEN TOP (Sodium Heparin*) tubes

  • *If sodium heparin blood collection tubes are unavailable, lithium heparin or ACD solution A can be used. Please contact the laboratory at 206-606-7700 for further instruction.

Alternate Specimens: Buccal Swabs or Saliva. Contact Fred Hutch CIL Lab at (206) 606-7700 to obtain collection kits.

Resource: FHCC CIL HLA Specimen Collection and Storage Requirements

Forms & Requisitions
  • FHCC Clinical Immunogenetics Laboratory HLA Requisition
Quantity
Requested: 10 mL blood in RED TOP plus 30 mL NaHep blood
Minimum: 10 mL blood in RED TOP plus 20 mL NaHep blood

Processing

Receiving Instructions

Hold and transport whole blood samples at ambient temperature.

  • Package samples with the accompanying requisition.
  • Send samples as received via Delivery Express courier to FH CIL (see open hours noted below). Include the full address and phone number on the package:
    • Fred Hutch Clinical Immunogenetics Laboratory (CIL)
    • 188 East Blaine Street
    • Suite 250, Room 2120
    • Seattle, WA 98102
    • Phone: (206) 606-7700
  • FH CIL accepts samples Monday through Thursday between 8:30 a.m. & 5:00 p.m. Samples sent on Fridays must be received before 2:30 p.m.
  • The lab is closed on weekends and holidays. Samples collected after hours, weekends, or holidays can be stored at room temperature and delivered to CIL the next working day.
  • Notify the CIL of incoming STAT orders by calling (206) 606-7700.

Stability: It is preferred for samples to arrive at FH CIL within 24 hours of collection. Samples are stable up to 3 days after collection.

Misc Sendout

Performance

Lab Department
FHCC Lab - Misc(SAM)
Frequency
Performed: Monday through Friday, 8 am to 5 pm.
Available STAT?
Yes, by arrangement. Call (206) 606-7700 to schedule STAT testing.
Performing Location(s)
FHCC FHCC Clinical Immunogenetics Laboratory (CIL)
206-606-7700

188 East Blaine Street
Suite 250, Room 2120
Seattle, WA 98102

Fax: (206) 606-1169
Email: cillabco@seattlecca.org
Hours: Samples are accepted Monday through Thursday between 8:30 a.m. an 5:00 p.m. Friday samples must be received before 2:30 p.m. The lab is closed on weekends and holidays.

Billing & Coding

LOINC
30896-5
Interfaced Order Code
UOW3709