Extended Respiratory Virus PCR Panel

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General Information

Lab Name
Resp Virus Semi-Quant PCR
Lab Code
REVSQT
Epic Ordering
Respiratory Virus Panel, Semi-Quant PCR
Description

The UW LDT respiratory panel is a multiplexed semi-qualitative real-time PCR (RT-PCR) assay intended for the detection and identification of multiple respiratory viral pathogens obtained from respiratory samples of individuals suspected of lower respiratory tract infections.

The following viruses are targeted:

  • Respiratory Syncytial Virus, RSV (matrix)
  • Metapneumovirus, MPV (fusion)
  • Influenza type A (matrix)
  • Influenza type B (matrix)
  • Parainfluenza type 1, PIV1 (polymerase)
  • Parainfluenza type 2, PIV2 (polymerase)
  • Parainfluenza type 3, PIV3 (matrix)
  • Parainfluenza type 4, PIV4 (nucleoprotein)
  • Coronavirus (polymerase) NOT SARS_CoV-2
  • Rhinovirus (5’NCR)
  • Bocavirus (NP1)
  • Adenovirus (hexon/penton)

This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration.

For ordering individual battery components:

FUSAMR, see Adeno/Metapneumo/Rhino Virus PCR Panel, [FUSAMR]

FLURSV, see Influenza A/B & RSV PCR Panel, [FLURSV]

FUSPIV, see Parainfluenza PCR Panel, [FUSPIV]

FUSCOV, Coronavirus (Non SARS-CoV-2) PCR Panel [FUSCOV]

Synonyms
adenoviruses, and human bocavirus, coronaviruses, FluA, FluB, Influenza A and B by PCR, MPV, PIV1-4, Respiratory Virus PCR, rhinoviruses, RSV
Components
Code Name
RSQSD Resp Virus PCR Spec
REVMPV Metapneumovirus PCR Qual Rslt
CTMPV Metapneumovirus Ct Value
REVP1 Parainfluenza 1 PCR Qual Rslt
CTP1 Parainfluenza 1 Ct Value
REVP2 Parainfluenza 2 PCR Qual Rslt
CTP2 Parainfluenza 2 Ct Value
REVP3 Parainfluenza 3 PCR Qual Rslt
CTP3 Parainfluenza 3 Ct Value
REVP4 Parainfluenza 4 PCR Qual Rslt
CTP4 Parainfluenza 4 Ct Value
REVRSV RSV PCR Qual Result
CTRSV RSV Ct Value
REVFLA Influenza A PCR Qual Result
CTFLA Influenza A Ct Value
REVFLB Influenza B PCR Qual Result
CTFLB Influenza B Ct Value
REVCV Coronavirus PCR Qual Result
CTCV Coronavirus Ct Value
REVRHN Rhinovirus PCR Qual Result
CTRHN Rhinovirus Ct Value
REVADV Adenovirus PCR Qual Result
CTADV Adenovirus Ct Value
REVBOC Bocavirus PCR Qual Result
CTBOC Bocavirus Ct Value
RSQINT Resp Virus Semi-Quant Interp

Interpretation

Method

Real-time Polymerase Chain Reaction.

Reference Range
See individual components

Ordering & Collection

Specimen Type
BAL, Washing, Swab, Sputum, or Aspirate. Note: Need approval from Laboratory Medicine Resident for Serum, Plasma, CSF, Body Fluid, or Tissue.
Collection

BAL, Washing, Swab, Sputum, or Aspirate: 1 mL in sterile tube.

Serum, Plasma, CSF, or Body Fluid: 1 mL in sterile tube.

Tissue: Pea size amount in sterile tube or universal transport media (UTM).

Minimum sample requirement: 0.5 mL

Handling Instructions

Refrigerate within 4 hours of collection. Send refrigerated specimen at 2 to 8°C to lab on ice packs within 48 hours of collection. Specimens frozen at -20°C are acceptable on dry ice.

Quantity
requested: 1 mL
minimum: 0.5 mL

Processing

Processing

Send sample to UW Virology Laboratory within 4 hours of collection. If sample cannot arrive within 4 hours of collection, store sample at 2-8°C (ice pack) for up to 48 hours of collection. If there is a greater than 48 hour delay before testing sample, freeze sample at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Note: If both REVSQT & testing for CVIR is ordered on a single specimen, the specimen must be split and an aliquot sent to each of the different laboratories. If splitting the sample is not possible, send sample to CVIR first with a note for CVIR to forward to EVIR. Minimum of 1 mL for EVIR test order.

Performance

LIS Dept Code
Virology, Eastlake (EVIR)
Performing Location(s)
Other Virology, Molecular
206-685-6656

Virology Laboratory, Ste 320
1616 Eastlake Ave E.
Seattle, WA 98102
Frequency
Monday – Saturday. Results same day.
Available STAT?
No

Billing & Coding

CPT codes
LOINC
50219-5
Interfaced Order Code
UOW2516