Trypanosoma cruzi Antibody Panel (Sendout)
General Information
- Lab Name
- Trypanosoma cruzi Antibody Panel (Sendout)
- Lab Code
- RCRUZI
- Epic Name
- Trypanosoma cruzi Antibody Panel (Sendout)
- External Test Id
- CRUZI
- Description
Useful For: Diagnosis of chronic Trypanosoma cruzi infection (Chagas disease) using two different methods.
Ordering Guidance: This panel includes two independent T. cruzi antibody assays performed using different methods, as recommended by the CDC for serologic diagnosis of Chagas disease¹. If results are discordant, additional testing may be required for resolution. Contact the Laboratory Medicine Resident or Microbiology fellow on-call for guidance on subsequent testing.
- References
- Centers for Disease Control and Prevention (CDC): Laboratory Diagnosis of American Trypanosomiasis
- Mayo Clinic Labs Clinical & Interpretive Information: Trypanosoma cruzi (Chagas) Antibody Panel, Serum
- Synonyms
- American trypanosomiasis, Chagas, Chagas disease, CRUZI, Kissing bug, RCHAGS, Reduviid, T. cruzi, Triatoma, Trypanosoma, Trypanosomiasis
- Components
-
Code Name RCRUZT Trypanosoma cruzi Total Antibody, EIA RCRUZG Trypanosoma cruzi IgG, LFA RCRZIN Trypanosoma cruzi Interpretation
Interpretation
- Guidelines
- Method
Enzyme-Linked Immunosorbent Assay (ELISA), Lateral Flow Assay (LFA)
- Reference Range
- See individual components
- Ref. Range Notes
Reference Values:
Trypanosoma cruzi Total Antibody, EIA: Negative Trypanosoma cruzi IgG, LFA: Negative Additional interpretive information is available on the Mayo website.²
- Interferences and Limitations
Cautions:
False-positive results may occur in patients infected with Leishmania species or other Trypanosoma species, including Trypanosoma rangeli. Additionally, false-positive results with the T cruzi lateral flow assay have been detected in patients with hepatitis C, toxoplasmosis, or syphilis.
A diagnosis of chronic Chagas disease requires both clinical evaluation (including exposure history) and laboratory results. Chagas disease should not be diagnosed based on a single serologic result alone.
A single negative result does not exclude the diagnosis of Chagas disease as antibodies to the pathogen may not yet be detectable. Sensitivity of the assay may be decreased in significantly immunosuppressed patients.
- References
- Centers for Disease Control and Prevention (CDC): Laboratory Diagnosis of American Trypanosomiasis
- Mayo Clinic Labs Clinical & Interpretive Information: Trypanosoma cruzi (Chagas) Antibody Panel, Serum
Ordering & Collection
- Specimen Type
- Blood
- Collection
-
3 mL blood in RED or GOLD SST tube
- Handling Instructions
Outside Laboratories: Centrifuge sample and aliquot serum to a separate plastic vial. Freeze serum at -20°C while awaiting shipment. Transport on dry ice.
Stability: Frozen (Required): 14 days; Refrigerated: Unacceptable; Ambient: Unacceptable.
Reject Due To: Gross hemolysis, gross lipemia.
- Quantity
-
Requested: 0.7 mL serum
Minimum: 0.5 mL serum
Processing
- Receiving Instructions
Centrifuge sample and aliquot serum to a separate plastic vial. Freeze serum at -20°C.
Sendouts:
- Order Mayo Test: CRUZI
- Interfaced: Yes [Interface: 601; Worksheet: MAFZ]
Stability: Frozen (Required): 14 days; Refrigerated: Unacceptable; Ambient: Unacceptable.
Reject Due To: Gross hemolysis, gross lipemia.
- Misc Sendout
Performance
- Lab Department
- Sendouts Mayo Lab (FZ)(MAFZ)
- Frequency
- Performed: Monday. Reported: 1-8 days from sample receipt at Mayo Clinic Labs.
- Available STAT?
- No
- Performing Location(s)
-
Sendout Mayo Clinic Laboratories (Superior Drive)
800-533-17103050 Superior Drive NW
Rochester, MN 55901
Billing & Coding
- CPT Codes
- 86753x2
- Interfaced Order Code
- UOW6010
- Interfaced Result Code
- UOW6013