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Mpox Virus Qualitative PCR

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General Information

Lab Name
Mpox Virus Qualitative PCR
Lab Code
MPXQLT
Epic Name
Mpox Virus Qualitative PCR
Description

The detection of mpox virus DNA by real-time PCR amplification. The primers used in this PCR reaction amplify the F3L gene (Maksyutov et al., J Virological Methods 2016) and the probe hybridizes the target sequences between the primers that specifically identifies the PCR amplicon. The minimum virus level that gives a positive result in 95% of replicates is 328 copies/mL in either VTM/UTM or Aptima multitest tubes.

For all reference laboratory questions, contact Client Support Services (available 24/7):

Phone: (206) 520-4600 or 1 (800) 713-5198
Fax: (206) 520-4903
Email: commserv@uw.edu

Forms & Requisitions

UW Medicine Locations: Place order in Epic for test "Mpox Virus Qualitative PCR" (lab test code MPXQLT).

Outside Locations/Reference Laboratory Clients: Include a completed Virology requisition with the specimen and specify "Monkeypox Virus Qualitative PCR" in the "other requests/comments" area.

Synonyms
Orthopoxvirus; Monkeypox; MPX; Monkey Pox; Mpox
Components
Code Name
MPXSD Mpox Virus Qual PCR Specimen Type
MPXQLR Mpox Virus Qual PCR Result
MPXIN Mpox Virus Qual PCR Interpretation
MPXMTH Mpox Virus Method

Interpretation

Guidelines
Method

Laboratory developed PCR

Reference Range
See individual components

Ordering & Collection

Specimen Type
Swabs in UTM/VTM, CSF, Plasma (heparin is NOT an acceptable anticoagulant), Serum, Whole Blood, Urine, Breast Milk, Saliva
Collection

Acceptable Sample Types: Swabs in VTM (viral transport media) or UTM (universal transport media) or Aptima Multitest Swab Transport Media or Dry Swabs

  • Preferred Specimen Type: Swabs of skin lesion (LESION/PUSTULE/VESICLE/RECTAL/GENITAL) in VTM/UTM
  • Alternative Specimen Type: Swab in Aptima Multitest Swab Transport Media; Dry swabs of skin lesion; Rectal and/or genital swabs with VTM/UTM, dry swab, or Aptima Multitest Swab Transport Media; CSF; Urine; Serum/Plasma; Whole blood; Breast Milk

Collection Instructions: Swab the lesion vigorously, to ensure adequate sample is collected. DO NOT UNROOF LESION.

Forms & Requisitions

UW Medicine Locations: Place order in Epic for test "Mpox Virus Qualitative PCR" (lab test code MPXQLT).

Outside Locations/Reference Laboratory Clients: Include a completed Virology requisition with the specimen and specify "Monkeypox Virus Qualitative PCR" in the "other requests/comments" area.

Handling Instructions

Storage and Transport (Reference Laboratory Clients):

  • Swabs in UTM/VTM or Dry swab: Ship samples on dry ice. If -10°C or colder freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 7 days of collection. Samples stored at 2-8°C greater than 7 days old may be rejected. Do not freeze/thaw.
  • Swab in APTIMA Tube: Store and transport at ambient temperature within 30 days of collection.
  • Cerebrospinal fluid: 500µL CSF in a sterile container. Transport CSF on ice, store at 2 to 8°C for up to 7 days post collection. If there is a greater than 7 days delay before testing of the specimen, store specimen at -60 to -85°C. Do not freeze/thaw.
  • Serum/Plasma: Separate plasma/serum from whole blood within 24 hours of collection. Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 7 days of collection. Samples stored at 2-8°C greater than 7 days old may be rejected. Do not freeze/thaw.
  • Clear non-cellular fluid: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 7days of collection. Samples stored at 2-8°C greater than 7 days old may be rejected. Do not freeze/thaw.
  • Breast Milk: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 7days of collection. Samples stored at 2-8°C greater than 7 days old may be rejected. Do not freeze/thaw.

Ship Specimens to:

UW Medicine
Deptartment of Laboratory Medicine and Pathology
1959 NE Pacific St., Room NW220
Seattle, WA 98195
Ph: (206) 520-4600 or (800) 713-5198

Additional Information:

Additional information on ordering, collection, and shipment can be found at https://depts.washington.edu/uwviro/order/.

Quantity
Requested: 1.0 mL
Minimum: 0.5 mL

Processing

Receiving Instructions

Swabs in UTM/VTM: Store at 2 to 8°C for up to 7 days post collection and transport on ice. If there is a greater than 7 days delay before testing of the specimen, store specimen at -60 to -85°C. Do not freeze/thaw.

Swab in APTIMA tube: Transport under ambient conditions within 30 days.

Cerebrospinal/Body fluids: 500µL CSF/Body Fluid in a sterile container. Transport CSF/Body Fluid on ice, store at 2 to 8°C for up to 7 days post collection. If there is a greater than 7 days delay before testing of the specimen, store specimen at -60°C or colder. Do not freeze/thaw.

Serum/Plasma: Harvest Sera/Plasma from whole blood within 24 hours of collection. Sera/Plasma may be stored at 2-8°C (ice pack) for up to 7 days. Freeze at -10°C or colder. Do not freeze/thaw.

Send to UW Virology Lab via DLMP courier.

Misc Sendout

Performance

Lab Department
Virology, Eastlake(EVIR)
Frequency
Wednesdays, results same day.
Available STAT?
No
Performing Location(s)
Other Virology, Eastlake
206-685-6656

Virology Laboratory, Ste 320
1616 Eastlake Ave E.
Seattle, WA 98102

Billing & Coding

CPT Codes
87593
LOINC
100383-9
Regulatory Category
Laboratory-developed test
Appropriate use
Research use only
Interfaced Order Code
UOW4562