JC Virus by PCR Quantitative

General Information

Lab Name

JC Virus_PCR, Quant

Lab Code

JCVQN

Epic Name

JC Virus Quantitative PCR, Blood/CSF

Description

The detection and quantitation of JC Virus (JCV) by real-time PCR amplification. The primers used in this PCR reaction amplify the Large T antigen gene. The JC Virus probes are labeled with reporter dye FAM and quencher TAMRA. Quantitation of JC Virus DNA (copies/mL) is achieved by amplifying standard curves consisting of serial dilutions of a known number of DNA containing the JC Virus amplicons. The minimum virus level that gives a positive result in 95% of replicates is 500 copies/mL (2.70 log copies/mL).

This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration.

For Tissue Biopsy, Bone Marrow, Swab, or Cellular fluid (e.g. BAL or Washing), see JC Virus Qualitative PCR, Tissue [JCVQLT].

Urine, see JC Virus by PCR Quantitative, Urine [UJCVQN].

Synonyms

JC Virus, PML Virus, Progressive Multifocal Leukoencephalopathy virus

Components

Code	Name
JCVQNS	JC Virus_PCR Specimen, Quant
JCVQNR	JC Virus_PCR Result, Quant
JCVLOG	JCV DNA Copies/mL (Log 10)
JCVQNI	JC Virus_PCR Interp, Quant