- Lab Name
- BRCA1&2 Analysis
- Lab Code
- Epic Ordering
- BRCA 1&2 Analysis
BRCA1 & BRCA2 testing is useful for the evaluation of patients who are suspected to have hereditary breast and ovarian cancer. Testing is performed using next-generation sequencing to detect most mutations in BRCA1 and BRCA2. The assay completely sequences all exons of these genes AND detects large deletions, duplications and mosaicism.
For patients with a suspected hereditary cancer syndrome, including hereditary breast and ovarian cancer syndrome, see BROCA Cancer Risk Panel [BROCA].
- Forms & Requisitions
- BART, BRCA1&2 Sequencing, BRCA1&2 deletion and duplication testing
- Reference Range
- See individual components
Ordering & Collection
- Specimen Type
- Peripheral Blood, cultured cells from skin biopsy, purified DNA from peripheral blood or cultured cells
- 10 mL whole blood in LAVENDER TOP EDTA tube.
- Also acceptable: YELLOW TOP ACD tube, purified DNA from peripheral blood or cultured cells.
- Collection and transport: Obtain 2-4 mm punch biopsy of skin sample under sterile conditions and place in transport media (e.g. Alpha-MEM media, RPMI). Transport media can be supplied by the lab; call 206-598-4488 to request. If transport media is not available, the following media are acceptable alternatives if shipping time will not exceed 24 hours: lactated Ringer's solution, viral transport medium, or sterile saline. DO NOT USE formaldehyde, formalin, alcohol, or 5% dextrose, or tissue culture medium buffered with bicarbonate.
- (2) T23 or (1) T75 flask (minimum 1-T25 flask)*.
*Prenatal testing requires concomitant testing for maternal cell contamination (see Online Test Guide [[MCC]] for ordering and specimen requirements)
- Forms & Requisitions
- Handling Instructions
Ship specimen at room temperature for overnight delivery.
Blood specimens can be held for up to 7 days before shipping if refrigerated.
Ship specimens to:
UW MEDICAL CENTER
LABORATORY MEDICINE - GENETICS LAB
1959 NE PACIFIC ST, ROOM NW220
SEATTLE, WA 98195-7110
requested: Entire sample
minimum: 5 ml whole blood
Blood: Refrigerate whole blood
Unacceptable Conditions: Frozen or clotted specimens
Stability (collection to initiation of testing): Ambient: 5 days; Refrigerated: 7 days; Frozen: Unacceptable
Purified DNA: Refrigerate DNA specimens. Frozen is acceptable.
- LIS Dept Code
- Genetics (GEN)
- Performing Location(s)
Attention: Genetics Lab
Clinical lab, Room NW220
University of Washington Medical Center
1959 NE Pacific Street
Seattle, WA 98195
Tel: 206-598–6429 M–F (7:30 AM–4:00 PM)
Lab email: firstname.lastname@example.org
Tel (EXOME only): 206-543-0459
Rebecca Gaulin, email@example.com
Angela Jacobson, MS, LGC firstname.lastname@example.org
Sarah Paolucci, MA, MS, LGC, email@example.com
Jenna Huey, MS, LGC, firstname.lastname@example.org
Sandra Coe, MS,LGC, email@example.com
Dru Leistritz, MS, LGC, firstname.lastname@example.org (EXOME testing only)
Variant Review Scientist
Ankita Jhuraney, PhD
Colin C. Pritchard, MD, PhD
Brian H. Shirts, MD, PhD
Christina Lockwood, PhD, DABCC
Stephen Salipante, MD, PhD
Eric Konnick, MD, MS
Niklas Krumm, MD,PhD
Vera Paulson, MD, PhD
Jillian Buchan, PhD, FACMG
- Results within 4-6 weeks, once sample arrives in the laboratory.
- Available STAT?
Billing & Coding
- CPT codes
- Billing Comments
For billing information, see BRCA1&2 Analysis Billing
For pricing information, contact Client Support Services 206-520-4600 or 800-713-5198.
Billing and Insurance Pre-Authorization
We offer insurance pre-authorization services (preauthorization is only done for providers who are external to the UW system).
Email: email@example.com or call 1-855-320-4869 for more information.