T Cell Clonality by PCR
General Information
- Lab Name
- T Cell Clonality by PCR
- Lab Code
- TCRG
- Epic Ordering
- T Cell Clonality Analysis by PCR
- Description
Monoclonal populations of T lymphocytes can be identified by testing for T cell receptor gamma chain (TCRG) gene rearrangements using a multiplex polymerase chain reaction assay followed by fragment analysis by capillary electrophoresis. A polyclonal T cell population will produce a Gaussian distribution of PCR products on capillary electrophoresis, while a clonal process will produce one or two discrete peaks.
Sensitivity: This assay detects approximately 95% of T cell clones, provided that the clonal population represents at least 2-5% of the T cells in a specimen.
Hematopathology test request form found at Request Form
- Synonyms
- Gamma chain, T Cell Receptor
- Components
-
Code Name TCELSD T Cell Clonality PCR Specimen TCELRS T Cell Clonality PCR Result TCELIN T Cell Clonality PCR Interp TCREV T Cell Clonality Results Reviewed by: TCELM T Cell Clonality PCR Method TCRGRN T Cell Clonality Case Number
Interpretation
- Method
Polymerase Chain Reaction (PCR)/Capillary Electrophoresis
- Reference Range
- See individual components
- Guidelines
Ordering & Collection
- Specimen Type
- Blood, Bone Marrow, Tissue - Fresh, frozen or FFPE/PET (Blocks), Fluids, Slides, Sorted Cells
- Collection
-
Preferred:
Blood: 6mL in Lavender Top (EDTA)Bone Marrow: 1-2mL in Lavender Top (EDTA)
Tissue, Fluids or Sorted cells: Sterile container in RPMI
Formalin-fixed paraffin embedded tissue (FFPE/PET) block
Slides: 10 unstained
Accepted:
Green Tops (Heparin), while green tops are accepted for testing, there is documentation that heparin can interfere with some PCR assays.Extracted DNA from CLIA certified laboratory.
Formalin-fixed paraffin embedded tissue (FFPE/PET)
Tissue from curls cut from FFPE/PET blocks or stained cover-slipped slides
Unacceptable:
Specimens received beyond stability limit.
Specimens with low volume or inadequate WBC
Decalcified or unacceptable fixatives - Handling Instructions
- Deliver samples to Hematopathology as soon as possible.
- If sample is older than 48 hours, please store at 2-6°C upon receipt and send refrigerated.
- Samples stored at room temperature or refrigerated older than 7 days will be rejected.
- Frozen samples should be shipped on dry ice.
- Quantity
-
requested: Blood: 6mL EDTA/BMA: 1-2mL EDTA/Tissue Blocks: Adequate size to cut 10-20 curls/Slides: 10 unstained
minimum: Blood: 1mL EDTA/ BMA: 0.5mL EDTA/Tissue Blocks: 5 curls/Slides: 5 slides
Processing
- Processing
Send specimens to Hematopathology – Molecular department for processing.
The stability limit for Blood and Bone Marrow is 7 days, samples beyond this limit will be rejected.Blood and BMA must arrive in lab by 7am and tissue by 12pm Friday to be included on Tuesday run.
Performance
- LIS Dept Code
- Molecular Hematopathology (MHP)
- Performing Location(s)
-
Other Hematopathology, Molecular
206-606-7060UW Hematopathology Laboratory, G7-800
Fred Hutchinson Cancer Center
825 Eastlake Avenue E.
Seattle, WA 98109Hematopathology, Molecular hours:
7:00 am - 5:30 pm M-F
8:00 am - 4:30 pm Saturday, closed on Sunday, and with reduced staffing on major holidays - Frequency
- Tuesday and Thursday: Results available up to 5 days.
- Available STAT?
- No
Billing & Coding
- CPT codes
- 81342
- LOINC
- 21753-9
- Interfaced Order Code
- UOW2780