M. tuberculosis complex DNA Detection

General Information

Lab Name

Lab Code

TBCDNA

Epic Ordering

M. tuberculosis complex DNA Detection

Description

For more information on this test, to download a requisition form, and for a list of acceptable and unacceptable specimens, please refer to: Molecular Diagnosis Microbiology Section Website

Synonyms

AFB PCR, broad range AFB PCR, broad range Mycobacteria PCR, hsp65, M. tuberculosis complex, Molecular AFB, Molecular Mycobacteria, Molecular Mycobacterium, MTB, MTB PCR, MTBC, Mycobacteria PCR, Mycobacterial identification, Mycobacterium identification, Mycobacterium tuberculosis complex identification, Mycobacterium tuberculosis complex PCR, TB PCR, TBC, Tuberculosis PCR, universal AFB PCR, universal Mycobacterium PCR

Components

Code	Name
TBCRS	MTB complex PCR: Detection, hsp65
TBCID	MTB complex PCR: hsp65 Identification
TBCSI	MTB complex PCR: Specimen Description
TBCSPI	MTB complex PCR: External Identifier
TBCSR	MTB complex PCR: Special Requests
TBCSC	MTB complex PCR: Specimen Comments
TBCNAE	MTB complex PCR: Specimen DNA Extraction
TBCREV	MTB complex PCR: Pathologist Review
TBCME	MTB complex PCR: Method Note

Interpretation

Method

DNA extraction, nucleic acid purification, polymerase chain reaction (PCR), sequencing

Reference Range

See individual components

Guidelines

Ordering & Collection

Specimen Type: Tissue (Fresh or paraffin embedded), Fluid
Collection: Specimen should be collected into a DNA free container labeled with at least two identifiers.

Also accepted: E-swabs and UTM (Universal Transport medium); Formalin-fixed paraffin-embedded tissues (FFPE, PET); non-blood body fluids (e.g. CSF, pleural fluid, vitreous...etc) collected in Vacutainer tubes without preservative; LAVENDER top (EDTA) tube.

Unacceptable: Tissues free-floating in formalin; samples containing citrate, or heparin; Blood/Serum specimens; stool; swab/fluid collected in tube containing agar
Handling Instructions: Tissue specimens should be submitted and maintained on dry ice.
Quantity: requested: 1 mL Fluid; 1 cubic centimeter Tissue preferred
minimum: Diagnostic yield generally proportional to specimen size.

Processing

Processing: UWMC/HMC – store and send specimen refrigerated and freeze specimens at -20°C upon arrival in UW-ML Microbiology. If specimen storage and transport will exceed 8 hours, freeze at -20°C.

Freeze specimens at -20°C upon arrival

Outside Laboratories: Freeze specimens in sterile containers at -20°C. Transport all specimens on dry ice.
Transport Temperature

Performance

LIS Dept Code

Micro Molecular Diag (MMD)

Performing Location(s)

UW-MT	Microbiology, Molecular Diagnostics 206-520-4600 Clinical Lab, Room # NW177, University of Washington Medical Center, 1959 NE Pacific street, Seattle, WA 98195

Frequency

Preliminary results in 2 to 4 days. Testing initiated upon receipt of specimen, Monday-Friday.

Available STAT?

Billing & Coding

CPT codes: 87556
LOINC: 38379-4