M. tuberculosis complex DNA Detection

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General Information

Lab Name
M. tuberculosis complex DNA Detection
Lab Code
TBCDNA
Epic Ordering
M. tuberculosis complex DNA Detection
Description

For more information on this test, to download a requisition form, and for a list of acceptable and unacceptable specimens, please refer to: Molecular Diagnosis Microbiology Section Website

Synonyms
AFB PCR, broad range AFB PCR, broad range Mycobacteria PCR, hsp65, M. tuberculosis complex, Molecular AFB, Molecular Mycobacteria, Molecular Mycobacterium, MTB, MTB PCR, MTBC, Mycobacteria PCR, Mycobacterial identification, Mycobacterium identification, Mycobacterium tuberculosis complex identification, Mycobacterium tuberculosis complex PCR, TB PCR, TBC, Tuberculosis PCR, universal AFB PCR, universal Mycobacterium PCR
Components

Interpretation

Method

DNA extraction, nucleic acid purification, polymerase chain reaction (PCR), sequencing

Reference Range
See individual components
Guidelines

Ordering & Collection

Specimen Type
Tissue (Fresh or paraffin embedded), Fluid
Collection

Specimen should be collected into a DNA free container labeled with at least two identifiers.

Also accepted: E-swabs and UTM (Universal Transport medium); Formalin-fixed paraffin-embedded tissues (FFPE, PET); non-blood body fluids (e.g. CSF, pleural fluid, vitreous...etc) collected in Vacutainer tubes without preservative; LAVENDER top (EDTA) tube.

Unacceptable: Tissues free-floating in formalin; samples containing citrate, or heparin; Blood/Serum specimens; stool; swab/fluid collected in tube containing agar

Handling Instructions

Tissue specimens should be submitted and maintained on dry ice.

Quantity
requested: 1 mL Fluid; 1 cubic centimeter Tissue preferred
minimum: Diagnostic yield generally proportional to specimen size.

Processing

Processing

UWMC/HMC – store and send specimen refrigerated and freeze specimens at -20°C upon arrival in UW-ML Microbiology. If specimen storage and transport will exceed 8 hours, freeze at -20°C.

Freeze specimens at -20°C upon arrival

Outside Laboratories: Freeze specimens in sterile containers at -20°C. Transport all specimens on dry ice.

Transport Temperature

Performance

LIS Dept Code
Micro Molecular Diag (MMD)
Performing Location(s)
UW-MT Microbiology, Molecular Diagnostics
206-520-4600

Clinical Lab, Room # NW177,
University of Washington Medical Center,
1959 NE Pacific street, Seattle, WA 98195

Frequency
Preliminary results in 2 to 4 days. Testing initiated upon receipt of specimen, Monday-Friday.
Available STAT?
No

Billing & Coding

CPT codes
87556
LOINC
38379-4