M. tuberculosis complex DNA Detection
General Information
- Lab Name
- M. tuberculosis complex DNA Detection
- Lab Code
- TBCDNA
- Epic Ordering
- M. tuberculosis complex DNA Detection
- Description
For more information on this test, to download a requisition form, and for a list of acceptable and unacceptable specimens, please refer to: Molecular Diagnosis Microbiology Section Website
- Synonyms
- AFB PCR, broad range AFB PCR, broad range Mycobacteria PCR, hsp65, M. tuberculosis complex, Molecular AFB, Molecular Mycobacteria, Molecular Mycobacterium, MTB, MTB PCR, MTBC, Mycobacteria PCR, Mycobacterial identification, Mycobacterium identification, Mycobacterium tuberculosis complex identification, Mycobacterium tuberculosis complex PCR, TB PCR, TBC, Tuberculosis PCR, universal AFB PCR, universal Mycobacterium PCR
- Components
-
Interpretation
- Method
DNA extraction, nucleic acid purification, polymerase chain reaction (PCR), sequencing
- Reference Range
- See individual components
- Guidelines
Ordering & Collection
- Specimen Type
- Tissue (Fresh or paraffin embedded), Fluid
- Collection
-
Specimen should be collected into a DNA free container labeled with at least two identifiers.
Also accepted: E-swabs and UTM (Universal Transport medium); Formalin-fixed paraffin-embedded tissues (FFPE, PET); non-blood body fluids (e.g. CSF, pleural fluid, vitreous...etc) collected in Vacutainer tubes without preservative; LAVENDER top (EDTA) tube.
Unacceptable: Tissues free-floating in formalin; samples containing citrate, or heparin; Blood/Serum specimens; stool; swab/fluid collected in tube containing agar
- Handling Instructions
Tissue specimens should be submitted and maintained on dry ice.
- Quantity
-
requested: 1 mL Fluid; 1 cubic centimeter Tissue preferred
minimum: Diagnostic yield generally proportional to specimen size.
Processing
- Processing
UWMC/HMC – store and send specimen refrigerated and freeze specimens at -20°C upon arrival in UW-ML Microbiology. If specimen storage and transport will exceed 8 hours, freeze at -20°C.
Freeze specimens at -20°C upon arrival
Outside Laboratories: Freeze specimens in sterile containers at -20°C. Transport all specimens on dry ice.
- Transport Temperature
Performance
- LIS Dept Code
- Micro Molecular Diag (MMD)
- Performing Location(s)
-
UW-MT Microbiology, Molecular Diagnostics
206-520-4600Clinical Lab, Room # NW177,
University of Washington Medical Center,
1959 NE Pacific street, Seattle, WA 98195 - Frequency
- Preliminary results in 2 to 4 days. Testing initiated upon receipt of specimen, Monday-Friday.
- Available STAT?
- No
Billing & Coding
- CPT codes
- 87556
- LOINC
- 38379-4