Sirolimus by LCMSMS

General Information

Lab Name
Sirolimus by LCMSMS
Lab Code
SIROLG
Epic Name
Sirolimus Level
Description

The concentration of sirolimus in whole blood is determined by liquid chromatography-tandem mass spectrometry after protein precipitation. The reportable range is 1.0 to 700.0 ng/mL with imprecision of less than 11 %CV.

Synonyms
Rapamune, Rapamycin, Sirol
Components

Interpretation

Method

Whole blood protein precipitation. Analysis by liquid chromatography - tandem mass spectrometry.

Reference Range
See individual components
Ref. Range Notes

Whole blood trough Sirolimus concentrations of 5 - 15 ng/mL are effective in reducing the incidence of acute rejection in renal transplant patients when Sirolimus is used as part of a combined immunosuppressive regimen.

Ordering & Collection

Specimen Type
whole blood
Collection

3 mL blood in a Lavender top tube. Trough specimen preferred

Quantity
Requested: entire sample
Minimum: 1.5 mL whole blood for 3 mL Lavender Liquid EDTA (K3 EDTA); 0.5 mL whole blood for 3 mL Lavender Dry EDTA (K2 EDTA)

Processing

Receiving Instructions

Refrigerate whole blood, do not centrifuge.Frozen sample acceptable - Do not repeat freeze-thaw cycle

Outside laboratories: transport specimen at 2-8 oC

Stability: Room temp: 2d; Refrig: 5 d

Misc Sendout

Performance

Lab Department
Chemistry, University(CHU)
Frequency
Daily, specimen in UW chemistry lab by 1:00 pm results by 4:30pm.
Available STAT?
No
Performing Location(s)
UW-MT Chemistry, Special
206-520-4600

Clinical Lab, Room NW220,
University of Washington Medical Center,
1959 NE Pacific street, Seattle, WA 98195

Billing & Coding

CPT Codes
80195
LOINC
29247-4
Interfaced Order Code
UOW2750