Porphyrins (Total), RBC (Sendout)

General Information

Lab Name

Porphyrins (Total), RBC

Lab Code

RWBPOR

Epic Name

Porphyrins (Total), RBC (Sendout)

External Test Id

PEE

Description

Useful For: Establishing a biochemical diagnosis of erythropoietic protoporphyria and X-linked dominant protoporphyria.

Testing Algorithm: This test is recommended for screening patients for possible erythropoietic protoporphyria and X-linked dominant protoporphyria. In addition, it can be used for evaluation of iron-deficiency anemia and chronic lead intoxication. Testing begins with total erythrocyte porphyrins. If the result is below 80 mcg/dL, it is normal, and testing is complete.

If the total erythrocyte porphyrin value is 80 mcg/dL or above, the Protoporphyrins, Fractionated, RBC (Sendout) [RWBFPP] will automatically be performed at an additional charge. The fractionation test results include noncomplexed (free) protoporphyrin and zinc-complexed protoporphyrin.

Patient Preparation: Patient should abstain from alcohol for 24 hours.

Ordering Requirements: Include a list of medications the patient is currently taking in the Epic order comment.

References

Mayo Clinical & Interpretive Information: Porphyrins Evaluation, Whole Blood

Synonyms

EPP, Erythrocyte Porphyrin, Free Erythrocyte Porphyrin (FEP), or XDP), PEE, Protoporphyrins Total Erythrocytes, RBC Porphyrins, Red Blood Cell Porphyrins, X-linked Dominant Protoporphyria (XLDPP, XLEPP

Components

Code	Name
RWBPRR	Porphyrins (Total), RBC
RWBPRI	Porphyrins (Total) Interpretation, RBC

Interpretation

Method

Spectrofluorometric

Reference Range

See individual components

Ref. Range Notes

Reference Values:

Porphyrins, Total, RBC:

<80 mcg/dL

Interpretation: Abnormal results are reported with a detailed interpretation that may include an overview of the results and their significance, a correlation to available clinical information provided with the specimen, differential diagnosis, and recommendations for additional testing when indicated and available.

Interferences and Limitations

Cautions: Alcohol suppresses enzyme activity potentially leading to false-positive results if it is ingested within 24 hours of specimen collection.

References

Mayo Clinical & Interpretive Information: Porphyrins Evaluation, Whole Blood

Ordering & Collection

Specimen Type

Whole Blood

Collection

Preferred: 4 mL blood in DARK GREEN (Sodium Heparin) tube placed on wet ice immediately after collection

Also Accepted (on ice): Dark green (lithium heparin), lavender (EDTA) top. Unspun Lime green PST tubes are also acceptable.

Place sample on wet ice after collection and expedite transport to the lab.

Handling Instructions

Outside Laboratories:

Keep sample on wet ice until it can be refrigerated. Refrigerate whole blood while awaiting transport. Ship with a cold pack to maintain proper temperature.
Include a list of medications that the patient is currently taking on the DLMP requisition.*
- *Use client-specific requisition when available. To obtain a client-specific requisition, contact Client Support Services via email at: commserv@uw.edu.

Stability: Refrigerated (required): 7 days; Frozen: Unacceptable; Ambient: Unacceptable.

Quantity

Requested: 4 mL whole blood
Minimum: 3 mL whole blood

Processing

Receiving Instructions

Refrigerate unspun whole blood as soon as possible. Keep sample on wet ice until it can be transferred to the refrigerator.

Sendouts:

Order Mayo Test: PEE
Interfaced: Yes [Interface: 601; Department: MARF]
If a list of patient's current medications is included (in the Epic order comment or on the DLMP requisition), edit the MayoAccess order before closing to enter that information in the Performing Lab Notes. Alternatively, print a copy of the Epic/DLMP requisition and package it with the associated Mayo Batch List.

Stability: Refrigerated (required): 7 days; Frozen: Unacceptable; Ambient: Unacceptable.

Misc Sendout

Performance

Lab Department

Sendouts Mayo Lab (RF)(MARF)

Frequency

Available STAT?

Performing Location(s)

Sendout	Mayo Clinic Laboratories 800-533-1710 200 First Street Southwest Rochester, MN 55901

Billing & Coding

CPT Codes: 84311
LOINC: 2814-2
Interfaced Order Code: UOW2136