Prothrombin (F2) G20210A Variant (Sendout) [Expedited Living Donor Workup]

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General Information

Lab Name
Prothrombin (F2) G20210A Variant (Sendout)
Lab Code
RPROTM
Epic Name
Prothrombin (F2) G20210A Variant (Sendout)
External Test Id
0056060
Description

Test Information: Detects prothrombin NM_000506.4 c.20210G>A *97 G>A (legacy nomenclature: G20210A) pathogenic variant.

Ordering Restrictions: This test is approved for UWMT Liver Transplant Clinic only and should be used exclusively for screening liver donor candidates. For orders from other locations, refer to in-house test Prothrombin DNA Screen [PRODS].

References
  1. ARUP Technical Information: Prothrombin (F2) c.*97G>A (G20210A) Pathogenic Variant
Synonyms
0056060, F2 DNA assay, Factor II (PT) 20210 Variant, Prothrombin (F2) c.*97G>A (G20210A) Pathogenic Variant, Prothrombin Gene Analysis
Components
Code Name
RPROTS Prothrombin (F2) G20210A Specimen
RPROTV Prothrombin (F2) G20210A Variant Result

Interpretation

Method

Polymerase Chain Reaction/Fluorescence Monitoring

Reference Range
See individual components
Ref. Range Notes
Result: Variant(s) Detected: Interpretation:
Negative No copies of the variant detected Does not exclude other hereditary risk factors.
Heterozygous One copy of the variant detected Confers a two- to fourfold increase in thrombotic risk in the absence of other risk factors¹
Homozygous Two copies of the variant detected Confers an increased thrombotic risk (not well characterized at this time)
Interferences and Limitations

Limitations:

  • Diagnostic errors can occur due to rare sequence variations.
  • F2 gene variants other than NM_000506.4 c.20210G>A, *97 G>A will not be detected.
References
  1. ARUP Technical Information: Prothrombin (F2) c.*97G>A (G20210A) Pathogenic Variant

Ordering & Collection

Specimen Type
Blood
Collection

3 mL in LAVENDER TOP (EDTA) tube

Also Accepted: Yellow (ACD Solution A or B), Pink (K2EDTA)

Approval Required
Approved for UWMC Liver Tranplant Clinic only as part of an expedited living donor workup.
Handling Instructions

Outside Laboratories: This test is available to UW Medicine providers only. Refer to Prothrombin DNA Screen [PRODS] for testing available within DLMP.

Quantity
Requested: 3 mL whole blood
Minimum: 1 mL whole blood

Processing

Receiving Instructions

Refrigerate whole blood.

Sendouts:

  • Order ARUP Test: 0056060.
  • Interfaced: Yes. [Interface: 602; Department: ARPR]

Stability: Refrigerated (preferred): 1 week; Ambient: 72 hours, Frozen: 1 month.

Unacceptable Conditions: Plasma or serum; collection of specimen in sodium heparin tubes.

Misc Sendout

Performance

Lab Department
Sendouts ARUP Lab (RF)(ARPR)
Frequency
Performed: Sunday - Saturday. Reported: 2-5 days.
Available STAT?
No
Performing Location(s)
Sendout ARUP Laboratories, Inc.
800-522-2787

500 Chipeta Way
Salt Lake City, UT 84108

Billing & Coding

CPT Codes
81240
LOINC
24475-6
Interfaced Order Code
UOW5917