Immune Function-PHA stimulated ATP

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General Information

Lab Name
Immune Fcn_PHA Stimulated ATP
Lab Code
RIMFCN
Epic Name
Immune Fcn - PHA Stimulated ATP
External Test Id
9000
Description

Clinical Utility: ImmuKnow is an immune cell function assay that detects cell-mediated immunity in an immunosuppressed population. The assay detects cell mediated immunity by measuring the concentration of ATP from CD4 cells following stimulation.

Ordering Note: Non-orderable battery included in the RIMFN package.

Synonyms
9000 ImmuKnow, Cylex, CylexT, IMMFCN, Immune Cell Function Assay
Components
Code Name
ATPLVL ATP in Stimulated CD4 Cells
FCNINT Cylex ImmuKnow Interpretation

Interpretation

Method

Procedure: ImmuKnow technology combines cell stimulation, cell selection, and quantification of metabolic markers (ATP) to measure cell-mediated immunity. ImmuKnow measures early response to stimulation by detecting intracellular ATP synthesis in CD4 cells selected from blood by monoclonal antibody-coated magnetic beads. The amount of ATP present in stimulated blood specimens is a measure of lymphocyte activity. Since the CD4 lymphocytes orchestrate cell-mediated immunity responses through immunoregulatory signaling, the measurement of CD4 activation reflects the degree of immune function.

Reference Range
See individual components
Ref. Range Notes
ATP Level Interpretation
< or = 225 ng/mL Low Immune Respose
226-524 ng/mL Moderate Immune Response
> or = 525 ng/mL Strong Immune Response

The ATP level ranges for ImmuKnow were established by testing 155 apparently healthy adults and 127 transplant recipients. A cumulative frequency of differences was used to select the ATP levels that give the best balance of results between immunosuppressed and non-immunosuppressed individuals. The cutoffs for the ATP level ranges are 225 and 525 ng/mL.

Ordering & Collection

Specimen Type
Blood
Collection

Collection Limitations:

  • Draw specimens Monday-Friday only, between 5AM and 1:00 PM. Specimens must be received in the lab by 1:30 PM for same day shipment to referral lab.
  • Do not draw on holidays or on the day prior to a holiday.
  • Expedite transport to the laboratory. Testing must be initiated by Eurofins Viracor within 30 hours of collection.

Collection:

  • 2-3 mL blood in Sodium Heparin GREEN TOP tube.
    • Unacceptable: Lithium Heparin GREEN TOP tube
Handling Instructions

Outside Laboratories: Due to the limited stability for these samples, outside facilities should arrange for testing directly with Eurofins Viracor.

Quantity
Requested: Entire sample
Minimum: 2 mL whole blood

Processing

Receiving Instructions
  • Maintain whole blood samples at room temperature; do not refrigerate or freeze.
  • Expedite processing. Notify sendouts staff on receipt of sample in lab.

Sendouts:

  • Order electronically in the Retriever system using Eurofins Viracor Test: 9000.
  • Send samples Monday - Friday only the same day as collection. Samples must arrive at Eurofins Viracor for setup the next day.
  • Package samples in the provided Eurofins Viracor Live Cell shipping kits. Affix the FedEx label to the outside of the box.
  • A dedicated Eurofins Viracor courier will pick up packages at the UW-MT lab window at approximately 3 PM.
  • Samples that miss the daily courier pickup must be sent via regular FedEx shipment.

Stability: Ambient: 30 hours; Refrigerated: Unacceptable; Frozen: Unacceptable.

Misc Sendout

Performance

Lab Department
Sendouts(SO)
Frequency
TAT: 36-48 hours from receipt of sample.
Available STAT?
No
Performing Location(s)
Sendout Eurofins Viracor
800-305-5198

18000 W. 99th Street
Lenexa, KS 66219

Billing & Coding

CPT Codes
86352
LOINC
53940-3