Bullous Pemphigoid BP180 and BP230 IgG Antibodies (Sendout)

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General Information

Lab Name
Bls. Pemph. Anti BP180, BP230
Lab Code
RBP12G
Epic Name
Bls. Pemph. Anti BP180, BP230
External Test Id
BPAB
Description

Useful for:

  • Initial screening in the diagnosis of pemphigoid and its variants.

Ordering Note: If request specifies “Pemphigoid Panel” or “Epithelial Basement Membrane Zone Antibodies," refer to separate test Bullous Pemphigoid Antibody Panel (Sendout) [RBPEMP].

References
Synonyms
BP 180, BP 230, BPAB, BPAG, BPAG1, BPAG2
Components
Code Name
R180BP Bullous Pemphigoid, Anti BP180
R230BP Bullous Pemphigoid, Anti BP230

Interpretation

Method

Enzyme-Linked Immunosorbent Assay (ELISA)

Reference Range
See individual components
Ref. Range Notes

Interpretation: Antibodies to bullous pemphigoid (BP) BP180 and BP230 have been shown to be present in most patients with pemphigoid. Adequate sensitivities and specificity for disease are documented and Mayo Clinic's experience demonstrates a very good correlation between BP180 and BP230 results and the presence of pemphigoid (see Supportive Data). However, in those patients strongly suspected to have pemphigoid, either by clinical findings or by routine biopsy and/or direct immunofluorescence, and in whom the BP180/BP230 assay is negative, follow-up testing by Cutaneous Immunofluorescence Antibodies [IgG], Serum is recommended.

Antibody titer may correlate with disease activity in some patients. Patients with severe disease may be expected to have high titers of antibodies to BP. Titers may decrease with clinical improvement.

Interferences and Limitations

Cautions: As with other diagnostic test procedures, the results obtained with bullous pemphigoid (BP) BP180 and BP230 enzyme-linked immunosorbent assay kit serve only as an aid to diagnosis and should not be interpreted as diagnostic in themselves.

Performance of the assay in pediatric patients has not been established.

Performance of the assay on other matrices besides serum has not been established.

References

Ordering & Collection

Specimen Type
Blood
Collection

3 mL blood in RED TOP tube or GOLD SST

Handling Instructions

Outside Laboratories: Centrifuge sample and transfer serum to a separate plastic vial. Refrigerate serum.

Stability: Refrigerated (preferred):14 days; Frozen: 30 days; Ambient: 14 days.

Quantity
Requested: 1 mL serum
Minimum: 0.5 mL serum

Processing

Receiving Instructions

Centrifuge sample and transfer serum to a separate plastic vial. Refrigerate serum.

Sendouts:

  • Order Mayo Test: BPAB.
  • Interfaced: Yes.

Stability: Refrigerated (preferred):14 days; Frozen: 30 days; Ambient: 14 days.

Misc Sendout

Performance

Lab Department
Sendouts Mayo Lab (RF)(MARF)
Frequency
Performed: Varies. Report Available: 1-5 days.
Available STAT?
No
Performing Location(s)
Sendout Mayo Clinic Laboratories
800-533-1710

200 First Street Southwest
Rochester, MN 55901

Billing & Coding

CPT Codes
83516x2
LOINC
9329-4
Interfaced Order Code
UOW3873