Adalimumab Level with Ab Reflex (Sendout)

Useful For: Therapeutic drug monitoring of adalimumab concentration.

Testing Algorithm: If the result is 8.0 mcg/mL or less, the Adalimumab antibody test will be performed at an additional charge.

***Note: Effective 02/12/2025, test results may be delayed due to a reagent shipping issue. Specimens may be sent out and will be stabilized at Mayo Clinic Laboratories until the normal testing schedule resumes. The duration of this delay is expected to last approximately 7-14 days.***

Cautions:

Tumor necrosis factor (TNF) measurement is not the analyte of choice for monitoring therapy with TNF inhibitors (such as adalimumab or infliximab), since TNF testing would not distinguish between free TNF and TNF bound to the monoclonal antibody, either in the extracellular or membrane-bound form of the cytokine.

Toxicity effects other than acute hypersensitivity infusion reactions have not been described nor correlated with high adalimumab concentrations.

Optimal therapeutic concentrations of adalimumab may vary according to the disease.* For adults with active inflammatory bowel disease, a concentration of 7.5 mcg/mL or greater is considered therapeutic.*

For patients taking biotin supplements, it is recommended to wait at least 12 hours after the last ingestion of biotin to collect a blood sample for this test.

*Refer to Mayo Clinical & Interpretive Information linked above.

Outside Laboratories: Centrifuge sample and transfer serum to a separate plastic vial. Refrigerate serum.

Stability: Refrigerated (preferred): 28 days; Frozen: 28 days, Ambient: Unacceptable.

Gross hemolysis and gross lipemia are acceptable.

Centrifuge sample and transfer serum to a separate plastic vial. Refrigerate serum.

Sendouts:

• Order Mayo Test: ADALX.
• Interfaced: Yes.

Stability: Refrigerated (preferred): 28 days; Frozen: 28 days, Ambient: Unacceptable.

Gross hemolysis and gross lipemia are acceptable.