CellFree DNA Prenatal Screen
General Information
- Lab Name
- CellFree DNA Prenatal Screen
- Lab Code
- PDNAS
- Epic Ordering
- Cell-Free DNA Prenatal Screen
- Description
This screening test evaluates the copy number state for all 46 chromosomes, including common aneuploidies (trisomy 13, 18, 21, and sex chromosomes). Individualized patient-provider discussion is highly recommended prior to ordering.
Ordering Requirements:
- Singleton Pregnancies:
- For orders submitted electronically via Epic, providers must complete the Clinical Order Questions in Epic or submit a completed UW Genetics Clinical Lab Request Form.
- Submissions from outside (reference lab) clients must be accompanied by a completed UW Genetics Clinical Lab Request form.
- Twin Pregnancies: For twin pregnancies, samples are sent out to Illumina. Refer to separate test #484, "Twin cfDNA Prenatal Aneuploidy Screen (Sendout)."
- A completed UW Genetics Clinical Lab Request form is required for outside (reference lab) orders.
- Singleton Pregnancies:
- Forms & Requisitions
- Synonyms
- cell-free fetal DNA, cfDNA, Down syndrome, fetal chromosome aneuploidy screen, NIPS, NIPT, noninvasive prenatal screening, noninvasive prenatal testing, Placental DNA, Trisomy 13, Trisomy 18, Trisomy 21, Turner syndrome
- Components
-
Code Name PDNAP cfDNA Panel PDNAX cfDNA Sex Chromosomes PDNAR cfDNA Result PDNAI cfDNA Interpretation PDNAF cfDNA Predicted Fetal Fraction PDNAC cfDNA Comment PDNAM cfDNA Method PDNAGC cfDNA Genetic Counselor PDNADI cfDNA Director
Interpretation
- Method
Massively parallel sequencing of total cell-free DNA (cfDNA)
- Reference Range
- See individual components
- Guidelines
Ordering & Collection
- Specimen Type
- Whole blood
- Collection
-
Two 10 mL whole blood in Streck Cell-Free DNA BCT® tubes (mottled black and tan).
Gently mix blood by inverting tube 8-10 times.
Do not send samples via pneumatic tube system. Samples collected in Streck tubes must be walked to the laboratory.
- Forms & Requisitions
- Handling Instructions
Walk samples to the laboratory after collection. Do NOT use the pneumatic tube system, as this may compromise the sample.
- Quantity
-
requested: 20 mL whole blood
minimum: 7 mL whole blood
Processing
- Processing
Note: For twin pregnancies, refer to test #484, Twin cfDNA Prenatal Aneuploidy Screen (Sendout).
Store whole blood at room temperature. Do not freeze. For orders not placed in Epic, a completed UW Genetics Clinical Lab Request form is required.
At the cfDNA Panel (PDNAP) prompt, enter PSFULL for "Full prenatal test with chromosomes 21, 18, 13 PLUS sex chromosome aneuploidies."
Enter PSAU for "Limited prenatal test for chromosomes 21, 18, 13 ONLY."
Stability: Ambient: 5 days
- Transport Temperature
Performance
- LIS Dept Code
- Genetics (GEN)
- Performing Location(s)
-
UW-MT Genetics Attention: Genetics Lab
Clinical lab, Room NW220
University of Washington Medical Center
1959 NE Pacific Street
Seattle, WA 98195Tel: 206-598–6429 M–F (7:30 AM–4:00 PM)
Fax: 206-598–0304
Lab email: genelab@uw.eduTel (EXOME only): 206-543-0459
Manager
Joe Bernal, jbernal@uw.edu
Genetic Counselors
Angela Jacobson, MS, LGC agibson@uw.edu
Sandra Coe, MS,LGC, scoe20@uw.edu
Dru Leistritz, MS, LGC, dru2@uw.edu (EXOME testing only)
Daniel W. Serber, PhD, MS, LCGC, dwserber@uw.eduVariant Review Scientist
Ankita Jhuraney, PhD
Sarah Paolucci, MA, MS, LGC, spaolucc@uw.edu
Catherine A. Darcey, MScFaculty
Colin C. Pritchard, MD, PhD
Brian H. Shirts, MD, PhD
Christina Lockwood, PhD, DABCC
Stephen Salipante, MD, PhD
Eric Konnick, MD, MS
Niklas Krumm, MD,PhD
Vera Paulson, MD, PhD
Jillian Buchan, PhD, FACMG - Frequency
- Turnaround time 8-10 business days
- Available STAT?
- No
Billing & Coding
- CPT codes
- 81420
- Billing Comments
81420
- LOINC
- 73967-2