NPM1 Quantitative for MRD
General Information
- Lab Name
- NPM1 Quantitative for MRD
- Lab Code
- NPM1NG
- Epic Name
- NPM1 Insertion Mutation by NGS
- Description
This quantitative test detects common insertion mutations in NPM1 gene and is intended for post-treatment minimal residual disease monitoring in NPM1-mutated AML. Purified genomic DNA is PCR amplified using double- indexed primers coupled with sequencing adaptors for subsequent next generation sequencing using an Illumina Miseq platform.
Reportable Range: 0.007 – 50.00%
Limit of Detection: 0.003%
Limit of Quantification: 0.007%
Precision near LOD (CV): 54%Hematopathology test request form can be found at Request Form.
- Synonyms
- Acute Myeloid Leukemia, AML, NPM, NPM-1, NPM1, NPM1 Exon 12 Insertion mutation, Nucleophosmin
- Components
-
Code Name NPM1SD NPM1 by NGS Specimen Descr. NPM1RS NPM1 by NGS Result NPM1MP NPM1 by NGS Mutation % NPM1V NPM1 by NGS Insertion Variant NPM1IN NPM1 by NGS Interpretation NPM1M NPM1 by NGS Method NPM1GR NPM1 by NGS Case Number
Interpretation
- Guidelines
- Method
PCR Amplification then Next Generation Sequencing
- Reference Range
- See individual components
Ordering & Collection
- Specimen Type
- Blood, Bone Marrow, Tissue - Fresh, frozen or FFPE/PET (Blocks), Fluids, Slides, Sorted Cells
- Collection
-
Preferred:
Blood: 6mL in Lavender Top (EDTA)Bone Marrow: 1-2mL in Lavender Top (EDTA)
Tissue, Fluids or Sorted cells: Sterile container in RPMI
Formalin-fixed paraffin embedded tissue (FFPE/PET) block
Slides: 10 unstained
Accepted:
Green Tops (Heparin), while green tops are accepted for testing, there is documentation that heparin can interfere with some PCR assays.Extracted DNA from CLIA certified laboratory.
Formalin-fixed paraffin embedded tissue (FFPE/PET)
Tissue from curls cut from FFPE/PET blocks or stained cover-slipped slides
Unacceptable:
Specimens received beyond stability limit.
Specimens with low volume or inadequate WBC
Decalcified or unacceptable fixatives - Forms & Requisitions
- Handling Instructions
- Deliver samples to Hematopathology as soon as possible.
- If sample is older than 48 hours, please store at 2-6°C upon receipt and send refrigerated.
- Samples stored at room temperature or refrigerated older than 7 days will be rejected.
- Frozen samples should be shipped on dry ice.
- Quantity
-
Requested: Blood: 6mL EDTA/BMA: 1-2mL EDTA/Tissue Blocks: Adequate size to cut 10-20 curls/Slides: 10 unstained
Minimum: Blood: 1mL EDTA/ BMA: 0.5mL EDTA/Tissue Blocks: 5 curls/Slides: 5 slides
Processing
- Receiving Instructions
Send specimens to Hematopathology – Molecular department for processing.
The stability limit for Blood and Bone Marrow is 7 days, samples beyond this limit will be rejected.Specimens must be received in lab by 7am on Wednesday to be included on run.
- Misc Sendout
Performance
- Lab Department
- Molecular Hematopathology(MHP)
- Frequency
- Performed once weekly on Wednesdays; results may take up to 10 days.
- Available STAT?
- No
- Performing Location(s)
-
Other Hematopathology, Molecular
206-606-7060UW Hematopathology Laboratory, G7-800
Fred Hutchinson Cancer Center
825 Eastlake Avenue E.
Seattle, WA 98109Hematopathology, Molecular hours:
7:00 am - 5:30 pm M-F
8:00 am - 4:30 pm Saturday, closed on Sunday, and with reduced staffing on major holidays
Billing & Coding
- CPT Codes
- 81310
- LOINC
- 54448-6
- Interfaced Order Code
- UOW3029