SARS-CoV-2 and Influenza A&B Qual PCR

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General Information

Lab Name
SARS-CoV-2 and Influenza A&B Qual PCR
Lab Code
Epic Ordering
SARS-CoV-2 and Influenza A&B Qual PCR

The UW Clinical Virology Laboratory in the Department of Laboratory Medicine and Pathology incorporates the modified FDA approved Panther Fusion® Flu A/B/RSV assay for the rapid detection and differentiation of human influenza A/B, and respiratory syncytial virus (RSV) using real-time PCR. The Hologic Emergency Use Authorization (EUA) SARS-CoV-2 Real-time RT-PCR assay is used for the detection of COVID-19 virus (SARS-CoV-2) RNA.

The Panther Fusion® Flu A/B/RSV assay targets the influenza A viruses (matrix gene), the influenza B viruses (matrix gene) and RSV (matrix gene). Results are reported as Detected or NOT detected and a semi-qualitative result is provided by reporting the PCR Cycle threshold (Ct) value for positive specimens.

The Hologic Emergency Use Authorization (EUA) SARS-CoV-2 Real-time RT-PCR assay targets two conserved regions of the SARS-CoV-2 (the causative agent for COVID-19) ORF1ab gene. The two regions are not differentiated; amplification of either or both regions is a presumptive positive (detectable) test result and amplification of neither target results a negative (non-detectable) test result





Reference Range
See individual components

Ordering & Collection

Specimen Type
Nasopharyngeal (NP) or Oropharyngeal (OP) Swab, Nasal swab

Acceptable Specimens and Media

We prefer nasopharyngeal or oropharyngeal swab in Universal Transport Media (UTM) or viral transport medium (VTM).

Acceptable specimen types include nasopharyngeal or oropharngeal swab in UTM/VTM, nasopharyngeal swabs in M4 or M6 media, nasal swabs in UTM/VTM.


Instructions for Nasopharyngeal Swab: Gently insert mini-tipped flocked nasopharyngeal swab (swab on flexible plastic shaft) through the nostril and into the nasopharynx, reaching the posterior nasopharynx. Leave swab in place for 2-3 seconds then rotate completely around for 10-15 seconds. Remove swab and repeat the same process in the other nostril with the same swab.

After the second swab is completed, immediately place into the sterile vial containing media (UTM is preferred). Make sure that the swab is fully immersed in media, and that the shaft is short enough to completely tighten the cap. Obtaining columnar epithelial cells will enhance reliability of viral detection.


Send to the laboratory as soon as possible. Sample may be stored at 2-8°C for up to 72 hours of collection. Ship immediately to lab at 2-8°C (ice pack). If collection to receipt in the lab will exceed 72 hours freeze at -10°C or colder and ship on dry ice. Do not freeze/thaw.

Important notes

Leaking samples will not be processed.
We currently cannot accept at-home collected swabs and await further FDA guidance on this issue.

Handling Instructions

Send specimens to

UW Medicine
Department of Laboratory Medicine
1959 NE Pacific Street, room NW220
Seattle, WA 98195
Tel: (206) 520-4600 or 1 (800) 713-5198

Additional information

Additional information on ordering, collection, and shipment can be found at

For all questions, contact Client Support Services (available 24/7):

Phone: (206) 520-4600 or 1 (800) 713-5198
Fax: (206) 520-4903

requested: 0.5mL
minimum: 0.5mL



Send sample to UW Virology Laboratory within 4 hours of collection. If sample cannot arrive within 4 hours of collection, store sample at 2-8°C (ice pack) for up to 72 hours of collection. If there is a greater than 72 hour delay before testing sample, freeze sample at -70°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Samples stored at 2-8°C greater than 72 hours old may be rejected. Do not freeze/thaw.

Transport Temperature


LIS Dept Code
Virology, Eastlake (EVIR)
Performing Location(s)
Other Virology, Renton

1601 Lind Ave SW
Renton, WA 98057

206-685-6656 opt 4

Test is performed multiple times a day; expect same-day results for specimens received in the morning, next-day otherwise.
Available STAT?

Billing & Coding

CPT codes