JAK2 V617F DNA Reflexive

Initial testing is performed to qualitatively evaluate for the JAK2 V617F mutation. If this is negative, Calreticulin exon 9 mutation [CALR] testing will be reflexively ordered (with additional charge). If CALR screening is also found to be negative, the MPL sequencing by NGS [HCAPSG] will be reflexively ordered (with additional charge). This assay does not evaluate for other JAK2 mutations including exon 12 nor other exons. If indicated, JAK2 single gene sequencing by NGS (HCAPSG) is suggested for evaluation of these potential variants and should be separately ordered.

Hematopathology test request form found at Request Form

**Due to staffing challenges, MPL turn-around-time is extended to 14 to 25 days; please call the lab if you have urgent testing needs”.

Preferred:
Blood: 6mL in Lavender Top (EDTA)

Bone Marrow: 1-2mL in Lavender Top (EDTA)

Tissue, Fluids or Sorted cells: Sterile container in RPMI

Slides: 10 unstained PB or BM aspirate smears

Accepted:
Green Tops (Heparin), while green tops are accepted for testing, there is documentation that heparin can interfere with some PCR assays.

Extracted DNA will only be accepted from CLIA certified laboratories.

Tissue from stained or cover-slipped slides

Unacceptable:
Specimens received beyond stability limit.
Specimens with low volume or inadequate WBC
Formalin-fixed paraffin embedded tissue (FFPE/PET)
Decalcified or unacceptable fixatives