Human Herpes 6 Virus Chromosome Integration and Typing
General Information
- Lab Name
- HHV6 Integration and Typing
- Lab Code
- HH6ABC
- Epic Ordering
- HHV6 Chromosome Integration and Typing
- Description
The detection and quantitation of Human Herpesvirus 6 (HHV-6) chromosomal integration and typing. Droplet Digital PCR partitions a single bulk reaction into ~20,000 individual reactions that are each read as positive or negative for DNA template to provide an absolute quantitation of DNA copies without the use of a standard curve. The result of HHV-6 copies/cell is resulted as a ratio obtained by (HHV-6 copies)/(RPP30 copies/2). If the HHV-6 virus to RPP30/2 cell ratio is approximately “one”, results are consistent with HHV-6 chromosomal integration of the virus into the patient cell genome.
This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration.
For HHV-6 Quantitation Testing, see HHV6 Quantitative by PCR [HH6QN].
- Synonyms
- HHV-6, HHV6, HHV6CI
- Components
-
Code Name CIHH6A HHV6 Type A CIHH6B HHV6 Type B HH6VCR HHV6 Virus to Cell Ratio HH6CII HHV6 Chromo Integrat Interp
Interpretation
- Method
Droplet Digital PCR
- Reference Range
- See individual components
Ordering & Collection
- Specimen Type
- Whole blood
- Collection
-
Blood: 5 mL blood in EDTA (LAVENDER TOP) tube. Send whole blood in room temperature within 24 hours of collection. DO NOT centrifuge, refrigerate, or freeze whole blood.
Also acceptable: 5 mL blood in PPT (PEARL TOP) tube
Unacceptable samples: GREEN TOP, RED TOP or GOLD SST tube. DO NOT centrifuge, refrigerate or freeze whole blood.
DNA Specimens: Extracted DNA specimens are acceptable given that their DNA concentration is between 50–100 ng/μL with a volume of at least 100 μL. Extracted DNA must be stored at -20/-80°C and transported to the lab frozen.
The lab only accepts isolated or extracted nucleic acid from CLIA-certified laboratories or laboratories meeting equivalent requirements as determined by CAP.
Minimum sample requirement: 1 mL whole blood
- Handling Instructions
Send whole blood specimens to the Lab immediately at room temperature.
The Laboratory MUST receive the sample within 48 hours of collection. If sample cannot get to the testing laboratory at room temperature within 48 hours, send whole blood at 2-8°C (ice pack).
Extracted DNA must be stored at -20°C to -80°C and transported to the lab frozen.
- Quantity
-
requested: 5 mL whole blood
minimum: 1 mL whole blood
Processing
- Processing
Blood: Hold whole blood at room temperature and send at room temperature. If sample cannot get to the testing laboratory at room temperature within 48 hours, send whole blood at 2-8°C (ice pack). DO NOT centrifuge, refrigerate, or freeze whole blood.
DNA Specimens: Extracted DNA specimens are acceptable given that their DNA concentration is between 50–100 ng/μl, with a volume of at least 100 μl. Extracted DNA must be stored at -20/-80°C and transported to the lab frozen.
Outside Laboratories: Send whole blood to laboratory at room temperature. If sample cannot get to the testing laboratory at room temperature within 48 hours, send whole blood at 2-8°C (ice pack). Samples greater than 48 hours old may be rejected.
Performance
- LIS Dept Code
- Virology, Eastlake (EVIR)
- Performing Location(s)
-
Other Virology, Molecular
206-685-6656Virology Laboratory, Ste 320
1616 Eastlake Ave E.
Seattle, WA 98102 - Frequency
- 2nd and 4th Wednesday of the month. Results same day.
- Available STAT?
- No
Billing & Coding
- CPT codes
- 87533x2
- LOINC
- 49391-6
- Interfaced Order Code
- UOW3056