Entero/parechovirus by PCR Qualitative

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General Information

Lab Name
Enterovirus and Parechovirus Qualitative PCR, Tissue/Cells
Lab Code
Epic Ordering
Enterovirus and Parechovirus Qualitative PCR, Tissue/Cells

This assay tests for the qualitative presence of parechovirus and enterovirus by RT-PCR detection of viral RNA. This assay detects Enterovirus D68. It does not distinguish which serotype of enterovirus or parechovirus is present. The enterovirus primers and probes target a 116 base fragment of the viral 5’ untranslated region. This region is the most conserved among >100 enterovirus genotypes. The parechovirus primers and probe target a 64 base fragment of the viral polyprotein. The amount of enterovirus/parechovirus required for detection in 95% of replicates is 500 viral copies/mL (2.70 log copies/mL). Results are reported out as “positive” (if cycle threshold is 40 or less) or “not detected”.

This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration.

Coxsackie virus, Echovirus, Enterovirus, HPeV, parechovirus



Real-time Polymerase Chain Reaction.

Reference Range
See individual components

Ordering & Collection

Specimen Type
Blood, CSF, BAL, Washing, Body fluid, Swab, Stool, or Tissue.

Blood: 3 mL blood in EDTA (LAVENDER TOP) tube.

CSF: 1 mL in sterile container.

BAL/Washing/Body fluid/Swab: 1 mL in sterile container.

Stool: 3-5 gm (minimum 1 gm)

Tissue: Pea Size in sterile tube or universal transport media (UTM).

Also Acceptable: 3 mL blood in GOLD SST, RED, or PPT (PEARL TOP) tube.

Unacceptable samples: Plasma collected in sodium or lithium heparin.

Note: Separate specimens MUST be submitted when both Viral Culture/Serology and PCR testing are ordered.

Minimum sample requirement: 0.5 mL

Handling Instructions

Refrigerate within 4 hours of collection. Send refrigerated specimen at 2 to 8°C to lab on ice packs within 48 hours of collection. Specimens frozen at -20°C are acceptable on dry ice.

requested: See Collection
minimum: 0.5 mL



Send sample to UW Virology Laboratory within 4 hours of collection. If sample cannot arrive within 4 hours of collection, store sample at 2-8°C (ice pack) for up to 48 hours of collection. Ship immediately at 2-8°C (ice pack). If time from collection may exceed 48 hours, freeze sample at -10°C or colder and ship on dry ice. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.


LIS Dept Code
Virology, Eastlake (EVIR)
Performing Location(s)
Other Virology, Molecular

Virology Laboratory, Ste 320
1616 Eastlake Ave E.
Seattle, WA 98102

Monday, Wednesday, Friday, results same day.
Available STAT?

Billing & Coding

CPT codes
87498, 87798
Interfaced Order Code