Protein Electrophoresis Reflexive Panel
General Information
- Lab Name
- Protein Electrophoresis Panel
- Lab Code
- ELPP
- Epic Name
- Protein Electrophoresis Reflexive Panel
- Description
Purpose: This test can be used to screen for and identify a monoclonal protein (M-spike).
The initial test performed is serum protein electrophoresis. Samples with a monoclonal band present on protein electrophoresis that has previously been identified in this laboratory will be resulted with the concentration of the known M-spike. Samples demonstrating previously unidentified protein bands of concern will be reflexively tested by immunofixation, which generates an additional result and charge. If a monoclonal band is identified by immunofixation, its concentration will be reported.
Serum free light chain testing (FLC) testing is available as a separate order, and is not included in this panel.- Synonyms
- Gammopathy, Immunofixation, M-Protein, MGUS, Monoclonal, Monoclonal antibody, Multiple myeloma, Myeloma, Serum electrophoresis, Serum protein electrophoresis, SPEP, Waldenstrom
- Components
-
Code Name ETP Protein (Total) EALB Albumin EA1 Alpha 1 EA2 Alpha 2 EB Beta EG Gamma INTRPE Electrophoresis Interp:
Interpretation
- Guidelines
- Method
Agarose Electrophoresis (AEP)
- Reference Range
- See individual components
- Interferences and Limitations
Hemolysis may falsely elevate alpha-2 and beta components.
Stability: 1 week refrigerated; stable long-term if frozen.
Ordering & Collection
- Specimen Type
- Blood
- Collection
-
3 mL blood in RED TOP or GOLD SST tube.
Pediatric: 2 full GOLD or RED TOP MICROTAINERS. - Quantity
- Requested: 0.5 mL serum ELP; 0.5 mL for TP
Processing
- Receiving Instructions
Refrigerate serum.
Make two aliquots: 0.5 mL to IMM for ELP and 0.5 mL to CHEM for ETP.OK to share ELP and IFIX on one tube if short sample. Forward shared pink slip to Immunology.
Outside Laboratory: If Total Protein is provided please free text "TP provided" after test ordered, and provide a copy of the slip to Panel Bench and Immunology.
- Misc Sendout
Performance
- Lab Department
- Immunology(IMM)
- Frequency
- Monday-Friday
- Available STAT?
- No
- Performing Location(s)
-
UW-MT Immunology
206-520-4600Clinical Lab, Room NW220,
University of Washington Medical Center,
1959 NE Pacific street, Seattle, WA 98195
Billing & Coding
- CPT Codes
- 84165x2
- LOINC
- 24351-9
- Interfaced Order Code
- UOW2008