Dysautonomia, Autoimmune/Paraneoplastic Evaluation, Serum (Sendout)
General Information
- Lab Name
- Lab Code
- 996
- External Test Id
- DYS2
- Description
Useful For:
- Investigating idiopathic dysautonomic symptoms
- Directing a focused search for cancer in patients with idiopathic dysautonomia
- Investigating autonomic symptoms that appear in the course or wake of cancer therapy and are not explainable by recurrent cancer or metastasis (detection of autoantibodies in this profile helps differentiate autoimmune dysautonomia from the effects of chemotherapy)
Test Algorithm: Refer to Mayo's algorithm (linked below) for additional detail on tests and potential reflex tests included in this panel. Reflex testing may be performed at additional charge.
- Synonyms
- AChR Ganglionic Neuronal Antibody, ANNA-1, Anti-CV2, Anti-Enteric Neuronal Antibody, Anti-Hu, Anti-Neuronal Nuclear Antibody Type 1, Antineuronal, AP3B2 IFA, Cantoxin (Receptor Antibodies), CASPR2 IgG CBA, Cerebellar Antibodies, Chorea, Collapsin Response-Mediator Protein-5 Antibody (CRMP-5), Cramp-fasciculation, CRMP-5 IgG, Dipeptidyl aminopeptidase-like protein 6, Dorsal Root Ganglion Antibody, DPPX Ab IFA, DYS2, Hu Antibody, Isaacs disease, LGI1 IgG CBA, Motor End-Plate Antibody, Motor Nerve Terminal Antibodies, Myoid Antibody, Neuromuscular hyperexcitability, Neuromyotonia, Neuronal ganglionic acetylcholine receptor antibody, Neuronal Nuclear Antibody Panel, Neuronal Potassium Channel Ab, Neuronal-Anti, Paraneoplastic Neurological Autoimmunity, PCA-2, Purkinje Cell Cytoplasmic Antibody Type 2, Stiff-man Syndrome
Interpretation
- Method
Panel tests and reflex tests use the following methods: Indirect Immunofluorescence Assay (IFA), Cell Binding Assay (CBA), Cell Binding Assay (CBA), Cell Binding Assay (CBA), Cell Binding Assay (CBA)
- Ref. Range Notes
Reference Values:
AChR Ganglionic Neuronal Antibody: < or =0.02 nmol/L Anti-Neuronal Nuclear Antibody, Type 1 (ANNA-1): Negative Adaptor Protein 3 Beta2 (AP3B2) Antibody IFA: Negative Contactin-Associated Protein-Like-2 (CASPR2) IgG Antibody CBA: Negative Collapsin Response-Mediator Protein-5 (CRMP-5) IgG WB: Negative Dipeptidyl Aminopeptidase-Like Protein 6 (DPPX) Antibody IFA: Negative Leucine-Rich Glioma Inactivated Protein-1 IgG (LGI1) Antibody CBA: Negative Purkinje Cell Cytoplasmic Antibody Type 2 (PCA-2): Negative Note: Includes reference values for panel tests only.
Neuron-restricted patterns of IgG staining that do not fulfill criteria for ANNA-1, CRMP-5-IgG, or PCA-2 may be reported as "unclassified anti-neuronal IgG." Complex patterns that include nonneuronal elements may be reported as "uninterpretable."
Note: CRMP-5 titers lower than 1:240 are detectable by recombinant CRMP-5 Western blot analysis. CRMP-5 Western blot analysis will be done on request on stored serum (held 4 weeks). This supplemental testing is recommended in cases of chorea, vision loss, cranial neuropathy, and myelopathy.
- Guidelines
Ordering & Collection
- Specimen Type
- Blood
- Collection
-
Patient Preparation:
- For optimal antibody detection, specimen collection is recommended before initiation of immunosuppressant medication or intravenous immunoglobulin treatment.
- This test should not be requested in patients who have recently received radioisotopes, therapeutically or diagnostically, because of potential assay interference. The specific waiting period before specimen collection will depend on the isotope administered, the dose given, and the clearance rate in the individual patient. Specimens will be screened for radioactivity prior to analysis. Radioactive specimens received in the laboratory will be held 1 week and assayed if sufficiently decayed, or canceled if radioactivity remains.
- Patient should have no general anesthetic or muscle-relaxant medications in the previous 24 hours.
Specimen Collection: Collect 12 mL blood in RED TOP tubes or GOLD TOP SST
- Handling Instructions
- Quantity
-
requested: 4 mL serum
minimum: 2.5 mL serum
Processing
- Processing
Centrifuge samples and transfer serum to a separate plastic vial. Refrigerate serum.
Login: SEND1-;REFRIG
- RSNDT1: MAYO
- RSTYP1: SRM
- RTSRQ1: ;Dysautonomia, Autoimmune/Paraneoplastic Evaluation, Serum (Mayo Test DYS2)
Sendouts, order Mayo Test: DYS2.
Stability: Refrigerated (preferred): 28 days; Frozen: 28 days; Ambient: 72 hours.
Reject Due To: Gross hemolysis, lipemia, or icterus.
Performance
- LIS Dept Code
- Performing Location(s)
-
Sendout Mayo Clinic Laboratories
800-533-1710200 First Street Southwest
Rochester, MN 55901 - Frequency
- Performed: Varies. Report Available: 7-10 days.
- Available STAT?
- No
Billing & Coding
- CPT codes
- Billing Comments
CPTs: 83519, 86255x7
If indicated, reflex testing may be performed at additional charge.
- LOINC