Y-PCR Analysis for Y Chromosome Microdeletions Associated with Male Infertility [UWMC Clinical Genomics Laboratory]

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General Information

Lab Name
Lab Code
565
Description

This test detects microdeletions of the Y chromosome, which are found in 5-13% of men who are otherwise healthy but have abnormal sperm shape or movement, a low sperm count, or no sperm. Testing is performed by UWMC - Montlake DLMP Clinical Genomics Laboratory.

Epic Ordering:

  • Test Name: YPCR.
  • Order Code: LABYPCR.
  • For orders submitted electronically via Epic, a separate requisition form is not required.
Synonyms
AZF, DAZ, DDX3Y, LABYPCR, Sertoli Cell Only Syndrome, USP9Y, Y Chromosome Deletion, Y Infertility, Y-PCR
Components

Interpretation

Method

This PCR-based analysis assesses 14 different regions along the length of the Y chromosome and will detect all of the microdeletions associated with male infertility, including the AZFa and AZFb/c regions. This analysis exceeds the minimum requirements outlined by the European Academy of Andrology (EAA) and the European Molecular Genetics Quality Network (EMQN).

Reference Range
Interferences and Limitations

Y chromosome microdeletion analysis via PCR will not detect single nucleotide variants or small intragenic deletions or duplications. Such variants have been reported but are a very rare cause of male infertility.

Ordering & Collection

Specimen Type
Extracted DNA, Peripheral Blood, Saliva
Collection

Keep samples at room temperature.

Peripheral Blood:

  • 3-4 mL in EDTA (lavender top vacutainer).

Extracted DNA:

  • 3-10 micrograms of DNA (minimum 1 microgram) at a concentration of 100 nanograms per microliter (or higher), suspended in water. We can only accept DNA isolated in a CLIA-certified laboratory or a laboratory meeting equivalent requirements as determined by the College of American Pathologists and/or the Centers for Medicare and Medicaid Services.

Saliva:

Forms & Requisitions

Outside Providers: CGL Constitutional Test Request Form

Handling Instructions

Outside Laboratories:

  • Sample collection kits are available upon request. Kits include sample collection tubes, a biohazard bag with absorbent material, a test requisition, and FedEx shipping materials. Contact the lab at 206.616.4062 to request a kit.
  • A completed request form must accompany all orders.
  • Samples are accepted Monday-Friday.
  • Ship sample to:
    • Clinical Genomics Laboratory
    • University of Washington Health Sciences Building
    • Room H-561
    • 1959 NE Pacific St
    • Seattle, WA 98195
Quantity
Requested: Varies; refer to Collection information.
Minimum: Varies; refer to Collection information.

Processing

Receiving Instructions

Hold samples at room temperature.

  • Call UWMC - Montlake DLMP Cytogenetics and Genomics Laboratory at 206.598.4489 for pick up. Cytogenetics is staffed on weekends to accept samples.
  • If no answer or after hours, call 206.598.4488 to leave message.
Misc Sendout

Performance

Lab Department
Frequency
Performed upon receipt of sample. TAT: 2-3 weeks
Available STAT?
Yes
Performing Location(s)
Pathology Clinical Genomics Laboratory
206-598-8684
Other Locations/Notes

Clinical Genomics Laboratory
University of Washington Health Sciences Building
Room H-561
1959 NE Pacific St
Seattle, WA 98195

Ph: (206) 616-4062

Billing & Coding

Billing Comments

CPT code: 81403

Prior Insurance Authorization: Contact gpab@uw.edu for details