Amphotericin B Level (Sendout)

Test Description: Amphotericin B levels are assayed by HPLC. The lowest limit of quantitation (LOQ) validated for amphotericin B by HPLC for this laboratory is 0.05 µg/ml. Levels can be measured following the administration of amphotericin B deoxycholate or a lipid formulation of amphotericin B (e.g., liposomal amphotericin B, amphotericin B lipid complex).

Ordering Requirements:

• A completed University of Texas Fungal Testing Laboratory Requisition must accompany all orders. Dosage information and date/time of last dose are required for testing.

3 mL blood in RED TOP or LAVENDER TOP (EDTA) tube

• Unacceptable: Gel barrier tubes

Outside Laboratories:

• Centrifuge sample and transfer serum or plasma into an amber/light-protected vial. Freeze serum/plasma at -20C while awaiting shipment. Transport on dry ice.
• Submit a completed UT Fungal Testing Laboratory Requisition along with the DLMP requisition. Dosage and date/time of last dose are required for testing.
  • *Use client-specific requisition when available. To obtain a client-specific requisition, contact Client Support Services via email at: commserv@uw.edu.

Stability: Frozen: Preferred; Refrigerated: Unacceptable; Ambient: Unacceptable.

Centrifuge sample and transfer serum or plasma into an amber/light-protected vial (Mayo T915). Freeze serum or plasma at -20C.

Login: SEND1-;FREEZE SERUM or PLASMA

• RSNDT1: UTSA
• RSTYP1: <Enter specimen type received>
• RTSRQ1: ;Amphotericin B Level (Mayo Test MML1085)

Sendouts:

• Order Mayo Test: ZW102
• Complete the "Order-at-Entry" questions in MayoAccess:
  
  • Performing Lab: University of Texas at San Antonio Fungal Testing Laboratory
  • Test Name: Amphotericin B Antifungal Drug Level
  • Test Code: MML1085
• Include the completed UT Fungal Testing Laboratory Requisition with the Mayo Batch Sheet.

Stability: Frozen: Preferred; Refrigerated: Unacceptable; Ambient: Unacceptable.