Antiretroviral Drug Level (Sendout)

Useful For: Therapeutic drug management for patients being treated with antiretroviral drugs. Trough concentrations (prior to next dose) are recommended for anti-HIV drugs.

Ordering Requirements: A completed University of Florida Infectious Disease Pharmacokinetics Laboratory (IDPL) Requisition must be submitted for all orders.

• For accurate interpretation of the result, the drug dose, number of doses per week, and date/time of last dose is required.
• Leave the billing section blank to be completed by lab staff. Completed requisitions can be uploaded to the Epic Media tab or accompany the patient to blood draw.

6 mL blood in plain RED TOP tube

• Submit a separate sample for each drug level requested.

Unacceptable: Gel tubes/SST

Outside Laboratories:

• Centrifuge sample and transfer serum to a separate plastic vial, leaving room at the top for expansion. Freeze serum at -70°C while awaiting shipment. Samples frozen at -20°C are also acceptable. Transport on dry ice.
• A completed University of Florida IDPL requisition should accompany the sample. Alternatively, include the following information on the DLMP requisition: drug dose, number of doses per week, date/time of last dose.

Stability: Frozen (Required): -70°C preferred, -20°C accepted; Refrigerated: Unacceptable; Ambient: Unacceptable. Do not allow samples to thaw.

Centrifuge sample and transfer serum to a separate plastic vial, leaving room at the top for expansion. Freeze serum at -70°C. Samples frozen at -20°C are also acceptable.

Note: Create a separate login for each drug ordered.

Login: SEND1-;FREEZE SERUM

• RSNDT1: MAYIDP
• RSTYP1: SRM
• RTSRQ1: [Select from options below]
  • ;Bictegravir Level (UF Test BIC)
  • ;Darunavir Level (UF Test DARU)
  • ;Dolutegravir Level (UF Test DTG)
  • ;Efavirenz Level (UF Test EFVL)
  • ;Lopinavir Level (UF Test LOPV)
  • ;Rilpivirine Level (UF Test RILP)

Sendouts:

• Order Mayo Test: ZW288 (Misc UF Health Path Lab) and result the "Test Level Questions" in MayoAccess with the appropriate UF Health test name and code (see table below).
• Include a completed UF Health IDPL test requisition with the MayoAccess batch sheet. Lab staff may fill out the requisition if the drug dosage, number of doses/week, and date/time of last dose are provided.

MayoAccess Test Level Questions:

Stability: Frozen (Required): -70°C preferred, -20°C accepted; Refrigerated: Unacceptable; Ambient: Unacceptable. Do not allow samples to thaw.