17-Hydroxypregnenolone (Sendout)

General Information

Lab Name

Lab Code

135

External Test Id

17OHP

Description

Useful For:

As an ancillary test for congenital adrenal hyperplasia (CAH), particularly in situations in which a diagnosis of 21-hydroxylase and 11-hydroxylase deficiency have been ruled out
Confirming a diagnosis of 3-beta-hydroxy dehydrogenase (3-beta-HSD) deficiency
Analysis for 17-hydroxypregnenolone is also useful as part of a battery of tests to evaluate females with hirsutism or infertility; both can result from adult-onset CAH.

References

Mayo 17-Hydroxypregnenolone, Serum Clinical & Interpretive Information
Kushnir MM, Rockwood AL, Roberts WL, et al: Development and performance evaluation of a tandem mass spectrometry assay for 4 adrenal steroids. Clin Chem 2006;52(8):1559-1567

Synonyms

17 Hydroxypregnenolone, 17 OH Pregnenolone, 17-Hydroxy-pregnenolone, 17-OH Pregnenolone, 17OHP, Hydroxypregnenolone

Interpretation

Method

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Ref. Range Notes

Reference Ranges, Children:²

Males:

Premature (26-28 weeks):	1,219-9,799 ng/dL
Premature (29-36 weeks):	346-8,911 ng/dL
Full term (1-5 months):	229-3,104 ng/dL
6 months-364 days:	221-1,981 ng/dL
1-2 years:	35-712 ng/dL
3-6 years:	<277 ng/dL
7-9 years:	<188 ng/dL
10-12 years:	<393 ng/dL
13-15 years:	35-465 ng/dL
16-17 years:	32-478 ng/dL

Tanner Stages:

Stage I:	<209 ng/dL
Stage II:	<356 ng/dL
Stage III:	<451 ng/dL
Stage IV-V:	35-478 ng/dL

Females:

Premature (26-28 weeks):	1,219-9,799 ng/dL
Premature (29-36 weeks):	346-8,911 ng/dL
Full term (1-5 months):	229-3,104 ng/dL
6 months-364 days:	221-1,981 ng/dL
1-2 years:	35-712 ng/dL
3-6 years:	<277 ng/dL
7-9 years:	<213 ng/dL
10-12 years:	<399 ng/dL
13-15 years:	<408 ng/dL
16-17 years:	<424 ng/dL

Tanner Stages:

Stage I:	<236 ng/dL
Stage II:	<368 ng/dL
Stage III:	<431 ng/dL
Stage IV-V:	<413 ng/dL

Reference Ranges, Adults:

Males:	> or =18 years: 55-455 ng/dL
Females:	> or =18 years: 31-455 ng/dL

Interferences and Limitations

Cautions: At birth, the hypothalamic-pituitary-adrenal axis and the hypothalamic-pituitary gonadal axis are activated and adrenal and sex steroid levels are high. In preterm infants, the elevations can be even more pronounced due to illness and stress. As a result, preterm infants may occasionally have 17-hydroxypregnenolone levels of up to 9,799 ng/dL. Term infants (0-28 days) will have levels <3,104 ng/dL. These then fall over the following 2 years to prepubertal levels of <277 ng/dL.

References

Mayo 17-Hydroxypregnenolone, Serum Clinical & Interpretive Information
Kushnir MM, Rockwood AL, Roberts WL, et al: Development and performance evaluation of a tandem mass spectrometry assay for 4 adrenal steroids. Clin Chem 2006;52(8):1559-1567

Ordering & Collection

Specimen Type: Blood
Collection: Preferred: 3 mL blood in RED TOP tube
Acceptable: GOLD SST
Handling Instructions: Outside Laboratories: Centrifuge sample and transfer serum to a separate plastic vial. Freeze serum at -20°C while awaiting shipment. Transport samples on dry ice.

Stability: Frozen (preferred): 28 days; Refrigerated: 28 days; Ambient: Unacceptable.
Quantity: requested: 1 mL serum
minimum: 0.5 mL serum

Processing

Processing

Centrifuge sample and transfer serum to a separate plastic vial. Freeze serum at -20°C

Login: SEND1-;FREEZE

RSNDT1: MAYO
RSTYP1: SRM
RTSRQ1: ;17 Hydroxypregnenolone (Mayo Test 17OHP)

Sendouts:

Order Mayo Test: 17OHP.

Stability: Frozen (preferred): 28 days; Refrigerated: 28 days; Ambient: Unacceptable.

Performance

LIS Dept Code

Performing Location(s)