Useful For: Evaluating patients with suspected autoimmune myelopathy, myelitis, paraneoplastic myelopathy using serum specimens. Refer to Autoimmune Encephalitis Testing Recommendations for additional ordering guidance.
Testing Algorithm: Reflex testing may be performed at additional charge. Refer to Mayo's algorithm linked below.
Patient Preparation:
Ordering Requirements: Laboratory Medicine Resident (LMR) approval is required.
Panel and reflex tests include the following methodologies: Indirect Immunofluorescence Assay (IFA), Radioimmunoassay (RIA), Western Blot (WB), Flow Cytometry (FCM); Cell-Binding Assay (CBA).
Reference Values:
Amphiphysin Antibody: | Negative |
Anti-Glial Nuclear Antibody Type 1 (AGNA-1): | Negative |
Anti-Neuronal Nuclear Antibody Type 1 (ANNA-1): | Negative |
Anti-Neuronal Nuclear Antibody Type 2 (ANNA-2): | Negative |
Anti-Neuronal Nuclear Antibody Type 3 (ANNA-3): | Negative |
Adaptor Protein 3 Beta2 (AP3B2) Antibody IFA: | Negative |
Collapsin Response-Mediator Protein-5 (CRMP-5) IgG Western Blot: | Negative |
Dipeptidyl Aminopeptidase-Like Protein 6 (DPPX) Antibody CBA: | Negative |
Gamma Aminobutyric Acid Receptor Type B (GABA-B-R) Antibody CBA: | Negative |
Glutamic Acid Decarboxylase (GAD65) Antibody Assay: | < or =0.02 nmol/L |
Glial Fibrillary Acidic Protein (GFAP) Antibody IFA: | Negative |
Metabotropic Glutamate Receptor 1 Antibody (mGluR1) Antibody IFA: | Negative |
Myelin Oligodendrocyte Glycoprotein Antibody (MOG) FACS: | Negative |
Neurochondrin Antibody IFA: | Negative |
Neuronal Intermediate Filament (NIF) Antibody IFA: | Negative |
Aquaporin-4 Receptor (NMO/AQP4) Antibody FACS: | Negative |
Purkinje Cell Cytoplasmic Antibody Type 1 (PCA-1): | Negative |
Purkinje Cell Cytoplasmic Antibody Type 2 (PCA-2): | Negative |
Septin-7 Antibody IFA: | Negative |
Tripartite Motif-containing Protein 46 (TRIM46) Antibody IFA: | Negative |
Note: Includes reference values for panel tests only.
Neuron-restricted patterns of IgG staining that do not fulfill criteria for ANNA-1, ANNA-2, ANNA-3, CRMP-5-IgG, PCA-1, or PCA-2 may be reported as "unclassified anti-neuronal IgG." Complex patterns that include non-neuronal elements may be reported as "uninterpretable."
Interpretation: A positive result is consistent with a diagnosis of autoimmune myelopathy in the appropriate clinical context.
Cautions:
Negative results do not exclude a diagnosis of autoimmune myelopathy.
Intravenous immunoglobulin (IVIg) treatment prior to the serum collection may cause a false-positive result.
12 mL blood in RED TOP tubes or GOLD SST
Outside Laboratories: Centrifuge sample and transfer serum to a separate plastic vial. Refrigerate serum.
Stability: Refrigerated (preferred): 28 days; Frozen: 28 days; Ambient: 72 hours.
Reject Due To: Gross hemolysis, gross lipemia, gross icterus.
Centrifuge sample and transfer serum to a separate plastic vial. Refrigerate serum.
Login: SEND1-;REFRIG
Sendouts:
Stability: Refrigerated (preferred): 28 days; Frozen: 28 days; Ambient: 72 hours.
Reject Due To: Gross hemolysis, gross lipemia, gross icterus.
Sendout |
Mayo Clinic Laboratories
800-533-1710 200 First Street Southwest |
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CPTs: 86255x16, 84182, 86341, 86363, 86053