Myelopathy, Autoimmune/Paraneoplastic Evaluation, Serum (Sendout)

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General Information

Lab Name
Lab Code
1071
External Test Id
MAS1
Description

Useful For: Evaluating patients with suspected autoimmune myelopathy, myelitis, paraneoplastic myelopathy using serum specimens. Refer to Autoimmune Encephalitis Testing Recommendations for additional ordering guidance.

Testing Algorithm: Reflex testing may be performed at additional charge. Refer to Mayo's algorithm linked below.

Patient Preparation:

  • For optimal antibody detection, specimen collection is recommended prior to initiation of immunosuppressant medication or intravenous immunoglobulin treatment.
  • This test should not be requested in patients who have recently received radioisotopes, therapeutically or diagnostically, because of potential assay interference. The specific waiting period before specimen collection will depend on the isotope administered, the dose given, and the clearance rate in the individual patient. Specimens will be screened for radioactivity prior to analysis. Radioactive specimens received in the laboratory will be held 1 week and assayed if sufficiently decayed or canceled if radioactivity remains.

Ordering Requirements: Laboratory Medicine Resident (LMR) approval is required.

Synonyms
Adaptor Protein 3 Beta2 Antibody, AGNA-1, Amphiphysin Antibody, ANNA-1, ANNA-2, ANNA-3, Anti-GFAP, Anti-Glial Nuclear Antibody Type 1, Anti-MOG, Anti-Neuronal Nuclear Antibody Type 1, Anti-Neuronal Nuclear Antibody Type 2, Anti-Neuronal Nuclear Antibody Type 3, AP3B2 IFA, Aquaporin-4 Receptor Antibody, Collapsin Response-Mediator Protein-5 IgG Western Blot, CRMP-5 IgG Western Blot, Dipeptidyl Aminopeptidase-Like Protein 6, DPPX Antibody CBA, GABA-B-R Antibody CBA, GAD65 Ab Assay, Gamma Aminobutyric Acid Receptor Type B, GFAP IFA, Glial Fibrillary Acidic Protein Antibody, Glutamic Acid Decarboxylase Antibody, MAS1, Metabotropic Glutamate Receptor 1 Antibody, mGluR1 Ab IFA, MOG FACS, Myelin Oligodendrocyte Glycoprotein Antibody, Neurochondrin IFA, Neuromyelitis optica, Neuronal Intermediate Filament Antibody, NIF IFA, NMO/AQP4 FACS, Paraneoplastic myelopathy, PCA-1, PCA-2, Purkinje Cell Cytoplasmic Antibody Type 1, Purkinje Cell Cytoplasmic Antibody Type 2, Septin-7 Antibody IFA, TRIM46 Antibody IFA, Tripartite Motif-containing Protein 46
Components

Interpretation

Guidelines
Method

Panel and reflex tests include the following methodologies: Indirect Immunofluorescence Assay (IFA), Radioimmunoassay (RIA), Western Blot (WB), Flow Cytometry (FCM); Cell-Binding Assay (CBA).

Reference Range
Ref. Range Notes

Reference Values:

Amphiphysin Antibody: Negative
Anti-Glial Nuclear Antibody Type 1 (AGNA-1): Negative
Anti-Neuronal Nuclear Antibody Type 1 (ANNA-1): Negative
Anti-Neuronal Nuclear Antibody Type 2 (ANNA-2): Negative
Anti-Neuronal Nuclear Antibody Type 3 (ANNA-3): Negative
Adaptor Protein 3 Beta2 (AP3B2) Antibody IFA: Negative
Collapsin Response-Mediator Protein-5 (CRMP-5) IgG Western Blot: Negative
Dipeptidyl Aminopeptidase-Like Protein 6 (DPPX) Antibody CBA: Negative
Gamma Aminobutyric Acid Receptor Type B (GABA-B-R) Antibody CBA: Negative
Glutamic Acid Decarboxylase (GAD65) Antibody Assay: < or =0.02 nmol/L
Glial Fibrillary Acidic Protein (GFAP) Antibody IFA: Negative
Metabotropic Glutamate Receptor 1 Antibody (mGluR1) Antibody IFA: Negative
Myelin Oligodendrocyte Glycoprotein Antibody (MOG) FACS: Negative
Neurochondrin Antibody IFA: Negative
Neuronal Intermediate Filament (NIF) Antibody IFA: Negative
Aquaporin-4 Receptor (NMO/AQP4) Antibody FACS: Negative
Purkinje Cell Cytoplasmic Antibody Type 1 (PCA-1): Negative
Purkinje Cell Cytoplasmic Antibody Type 2 (PCA-2): Negative
Septin-7 Antibody IFA: Negative
Tripartite Motif-containing Protein 46 (TRIM46) Antibody IFA: Negative

Note: Includes reference values for panel tests only.

Neuron-restricted patterns of IgG staining that do not fulfill criteria for ANNA-1, ANNA-2, ANNA-3, CRMP-5-IgG, PCA-1, or PCA-2 may be reported as "unclassified anti-neuronal IgG." Complex patterns that include non-neuronal elements may be reported as "uninterpretable."

Interpretation: A positive result is consistent with a diagnosis of autoimmune myelopathy in the appropriate clinical context.

Interferences and Limitations

Cautions:

Negative results do not exclude a diagnosis of autoimmune myelopathy.

Intravenous immunoglobulin (IVIg) treatment prior to the serum collection may cause a false-positive result.

Ordering & Collection

Specimen Type
Blood
Collection

12 mL blood in RED TOP tubes or GOLD SST

Approval Required
Laboratory Medicine Resident approval is required.
Handling Instructions

Outside Laboratories: Centrifuge sample and transfer serum to a separate plastic vial. Refrigerate serum.

Stability: Refrigerated (preferred): 28 days; Frozen: 28 days; Ambient: 72 hours.

Reject Due To: Gross hemolysis, gross lipemia, gross icterus.

Quantity
Requested: 4 mL serum
Minimum: 2 mL serum

Processing

Receiving Instructions

Centrifuge sample and transfer serum to a separate plastic vial. Refrigerate serum.

Login: SEND1-;REFRIG

  • RSNDT1: MAYO
  • RSTYP1: SRM
  • RTSRQ1: ;Myelopathy, Autoimmune Paraneoplastic Evaluation, Serum (Mayo Test MAS1)

Sendouts:

  • Order Mayo Test: MAS1.

Stability: Refrigerated (preferred): 28 days; Frozen: 28 days; Ambient: 72 hours.

Reject Due To: Gross hemolysis, gross lipemia, gross icterus.

Misc Sendout

Performance

Lab Department
Frequency
Performed: Monday - Sunday. Report Available: 10-13 days from sample receipt at performing lab.
Available STAT?
No
Performing Location(s)
Sendout Mayo Clinic Laboratories
800-533-1710

200 First Street Southwest
Rochester, MN 55901

Billing & Coding

Billing Comments

CPTs: 86255x16, 84182, 86341, 86363, 86053

  • Note: Includes panel tests only. Reflex testing may be added at additional charge.